CRIT Antibody | 4644

//www.bio-rad-antibodies.com/human-crit-antibody-4644-hca031.html
CRIT Antibody | 4644 gallery image 1

Western blot detection of human CRIT in podocyte cell extract. Note visualisation of target protein at approximately 36kD. HCA031 was detected using Goat anti-human IgG Fab (Product code 0500-0099)

Enlarge
  • CRIT Antibody | 4644 thumbnail image 1
  • Human anti Human CRIT
  • Human anti Human CRIT
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    4644
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA031E, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA031
    HCA031AE, WBdatasheet pdfdatasheet pdf50 µg
    HCA031A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Human CRIT antibody, clone 4644 recognizes the novel human complement regulatory cell surface receptor designated CRIT (Complement C2 receptor inhibitor trispanning). CRIT binds complement factor C2 and blocks the classical pathway of complement activation.

      CRIT protein expression has been specifcally identified by immunohistochemistry in podocyte cells in human kidney glomeruli, and is also expressed on hematopoietic cells and a range of other human tissues.

      CRIT appears to function as a regulatory molecule protecting autologous cells from damage by complement. Expression has also been demonstrated by human parasites, which have acquired CRIT by horizontal transmission (Inal, J.M. et al.).
    • Intended Use
    • Target Species
      Human
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 50 µgReconstitute with 0.5 ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 50 µgReconstitute with 0.5 ml distilled water
    • Preparation
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      None present.
      None present.
    • Immunogen
      Synthetic peptide derived from the first 27 amino acids of the first extracellular domain of human CRIT conjugated to BSA and Transferrin (MSPSLVSYTQKNERGSHEVKIKHFSPY).
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 0.1 mg/ml following reconstitution
      Antibody concentration 0.1 mg/ml following reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
    • Shelf Life
      6 months from date of reconstitution.
      6 months from date of reconstitution.
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/1001/500
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/1001/500

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CRIT Antibody Formats

    Formats Clone Applications Sizes available
    CRIT Antibody : Purified 4644 E, WB 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      MCA2200P
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...

              You May Also Be Interested In...