CREB1 Antibody | 3F1-1B2

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CREB1 Antibody | 3F1-1B2 gallery image 1

Staining of paraffin embedded human lymph node with Mouse anti Human CREB1:Azide Free (MCA3026Z)

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CREB1 Antibody | 3F1-1B2 gallery image 2

Western blot analysis of HepG2 human hepatocellular carcinoma whole cell lysate probed with Mouse anti Human CREB1 antibody (MCA3026Z) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence

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  • Mouse anti Human CREB1:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    3F1-1B2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3026ZP *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA3026Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human CREB1 antibody, clone 3F1-1B2 recognizes human CREB1, also known as Cyclic AMP-responsive element-binding protein 1 or CREB1 isoform A. CREB1 is a 341 amino acis, ~37kDa transcription factor, a member of the leucine zipper family of DNA binding proteins. CREB1 contains both a basic leucine zipper domain and a kinase inucible domain and is involved in the regulation of many diverse cellular functions (Wen et al. 2010).

      Mouse anti CREB1 antibody, clone 3F1-1B2 binds CREB1 isoform A, other isoforms of CREB1 exist, caused by alternative splicing. Binding of clone 3F1-1B2 with isoforms B and 3 has not been determined. Mutations of the CREB1 gene can occur and potentially result in severe developmental anomolies (Kitazawa et al. 2012).

      Mouse anti Human CREB1 antibody, clone 3F1-1B2 has been used successfully for the detection of human CREB1 in HepG2 cells by Western blotting.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting
      Immunohistology - Paraffin(1)0.110 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CREB1 Antibody Formats

    Formats Clone Applications Sizes available
    CREB1 Antibody : Preservative Free 3F1-1B2 P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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