Corticotropin Releasing Factor Receptor 1 Antibody

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Corticotropin Releasing Factor Receptor 1 Antibody gallery image 1

Western blotting analysis of human colon (A), cerebellum (B), rat brain (C) and mouse spinal cord (D) lysates (35μg protein in RIPA buffer) probed with Goat anti Human corticotropin releasing factor receptor 1 antibody (AHP1674) used at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

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Corticotropin Releasing Factor Receptor 1 Antibody gallery image 2

Confocal immunofluorescence image of neuronal cell dendrites within a C57 mouse cortex stained with Goat anti Human corticotropin releasing factor receptor 1 antibody (AHP1674) used at 0.3μgml-1 (green), counter-stained with DAPI (blue). Data kindly provided by Dr Y. Chen, TZ Baram lab, UC Irvine, CA.

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  • Corticotropin Releasing Factor Receptor 1 Antibody thumbnail image 1
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  • Goat anti Human Corticotropin Releasing Factor Receptor 1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1674E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1674
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human corticotrophin releasing factor receptor 1 antibody recognises Corticotropin releasing factor receptor 1 (CRF1), a transmembrane protein and member of the G-protein coupled receptor 2 family, which acts as a high-affinity receptor for corticotropin releasing factor (CRF), and therefore a crucial mediator of physiological and behavioural responses to stress factors.

      Goat anti Human corticotrophin releasing factor receptor 1 antibody (AHP1674) suitable for use in immunofluorescence studies.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to Corticotropin releasing factor receptor 1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Peptide sequence C-NEEKKSKVHYH from the internal region of Corticotropin releasing factor receptor 1 (NP_004373.2).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      female pregnancy
      corticotrophin-releasing factor receptor activity
      parturition
      immune response
      integral to plasma membrane
      G-protein signaling, coupled to cAMP nucleotide second messenger
      activation of adenylate cyclase activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/32000
      Western Blotting0.10.3ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1674 detects a band of approximately 60-70kDa in human cerebellum and colon cell lysates
    • Instructions For Use

    Additional Corticotropin Releasing Factor Receptor 1 Antibody Formats

    Formats Applications Sizes available
    Corticotropin Releasing Factor Receptor 1 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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