Complement Factor H Antibody (PrecisionAbTM Antibody)

Complement Factor H Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of human plasma lysates probed with complement factor H antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 60 second exposure. Arrow points to complement factor H (molecular weight 155 kDa)

  • Complement Factor H Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Goat anti Complement Factor H
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    Complement factor H antibody recognizes complement factor H, also known as H factor 1 (complement), H factor 2 (complement), adrenomedullin binding protein, age-related maculopathy susceptibility 1, beta-1 H-globulin, beta-1H, factor H or factor H-like 1. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00158WBdatasheet pdfdatasheet pdf100 µl
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      • Goat anti Human complement factor H antibody recognizes complement factor H, also known as H factor 1.

        CFH, the gene encoding complement factor H, is a member of the regulator of complement activation (RCA) gene cluster. Complement factor H contains twenty short consensus repeat (SCR) domains, is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in complement factor H have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Oct 2011).

        Goat anti Human complement factor H antibody detects a band of 155 kDa. The antibody has been extensively validated for western blotting using human plasma lysates.
      • Target Species
      • Western Blotting
        Anti complement factor H detects a band of approximately 155 kDa in human plasma samples.
      • Product Form
        Purified IgG - liquid
      • Preparation
        Goat polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3) 0.5% BSA
      • Immunogen
        Peptide with the sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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