Complement Factor H Antibody

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Published customer image:
Sheep anti Human complement factor H antibody (4400-9504) used as a detection reagent in a sandwich ELISA for HfH levels in sera with Mouse anti Human factor H antibody, clone OX-23 (MCA508G) as a capture reagent.
Image caption:
Effect of the presence of hfH in mice on the nOMV immunogenicity.
A. Evolution of hfH concentration in mouse serum over time after two IP injection of 0.5mg purified hfH (black arrows) 24 and 0.5 hours prior to immunization (grey arrow). B. Serum Bactericidal Assay (SBA) titers against 44/76-SL wild-type in pooled sera from C57bl/6 mice pre-injected with 800 µg/ml hfH or hSA, 14 days following immunization with 5 &mu:g of nOMVlpxl1 or nOMVdis. C. Individual SBA titers against 44/76-SL, dots represent individual animals D. Individual ELISA titers against rfHbp induced after one dose of H44/76Lpxl1 OMV in mice pre-injected with hfH or hSA, *p = 0.016, not all sera were available. C, D dots represent individual animals, the geometric mean and 95% confidence interval are represented as horizontal bars.

From: Daniels-Treffandier H, de Nie K, Marsay L, Dold C, Sadarangani M, Reyes-Sandoval A, et al. (2016)
Impact of Reducing Complement Inhibitor Binding on the Immunogenicity of Native Neisseria meningitidis Outer Membrane Vesicles.
PLoS ONE 11(2): e0148840.

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  • Sheep anti Human Complement Factor H
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    • Product Type
      Polyclonal Antibody
    • Isotype
      Polyclonal IgG
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      4400-9504E, IDdatasheet pdfdatasheet pdf1 ml
      4400-9504
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Sheep anti Human complement Factor H antibody recognizes human Factor H, a cofactor involved in the regulation of the alternative complement pathway.In IEP a single arc is observed against human plasma. Identity has been confirmed by double diffusion against human plasma and a known anti-human Factor H antibody. Deficiency in factor H can lead to diseases such as Hemolytic-uremic syndrome and chronic hypocomplementemic nephropathy.

        Sheep anti Human Complement Factor H antibody has been used successfully as a capture reagent for the measurement of murine factor H in mouse serum samples by sandwich ELISA in conjunction with Mouse anti Human Factor H antibody, clone OX-23 (MCA508G ) as a detection reagent (Daniels-Treffandier et al. 2016).
      • Intended Use
      • Target Species
        Human
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Goatno
        Guinea Pigyes
        Bovineno
        Dogyes
        Chickenno
        Horseyes
        Ratyes
        Rabbitno
        Mouseyes
        Catyes
        Pigno
        Bovineno
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Ig fraction - liquid
      • Reconstitution
      • Preparation
        Ig fraction prepared by ammonium sulphate precipitation and ion exchange chromatography.
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.1% EACA
        0.01% Benzamidine
        1mM EDTA
      • Immunogen
        Human Factor H, purified from plasma.
      • Purity
      • Approx. Protein Concentrations
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Glycine buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        Please see label for expiry date.
      • UniProt
      • Entrez Gene
      • Acknowledgements
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
        Immunodiffusion

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
      • Technical Advice
      • Recommended Protocol
      • ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Complement Factor H Antibody Formats

      Formats Applications Sizes available
      Complement Factor H Antibody : Purified E, ID 1 ml

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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                References

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