Collagen VI Antibody

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Collagen VI Antibody gallery image 1

Staining of formalin fixed, paraffin embedded human lung with Rabbit anti Human collagen VI (AHP2049)

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Collagen VI Antibody gallery image 2

Staining of formalin fixed, paraffin embedded human breast with Rabbit anti Human collagen 6 (AHP2049)

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  • Rabbit anti Collagen VI
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2049P *datasheet pdfdatasheet pdf50 µg
    AHP2049
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human collagen VI antibody detects mammalian collagen type VI, also known as COL6A, a secreted extracellular matrix protein involved in cellular adhesion.

      Collagen type specific antibodies are dependent on non-denatured three-dimensional epitopes. Therefore, the reactivity and selectivity of this antibody will be significantly reduced if denatured and reducing conditions of SDS-PAGE and immunblotting are used.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      MammalsExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to Collagen IV was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.01% Sodium Azide (NaN3)
    • Immunogen
      Collagen Type VI from Human and Bovine Placenta.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      0.125M Sodium Borate, 0.075 Sodium Chloride, 0.005M EDTA
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      platelet-derived growth factor binding
      axon guidance
      collagen type VI
      cell adhesion
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin(1)15 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human breast or lung
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Collagen VI Antibody Formats

    Formats Applications Sizes available
    Collagen VI Antibody : Purified P * 50 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          Histar Detection SystemSTAR3000C500 TestsC, P
          STAR3000C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human breast or lung

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