Collagen IV Antibody

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Goat anti Human collagen IV antibody used for the demonstration of rat retinal vasculature by immunofluorescence.
Image caption:
Intravitreal injection of STZ does not modify retinal vasculature. A. Glucose transporter GLUT-2 expression (RT-PCR) in newborn and adult retinas, as compared to adult pancreas. GLUT-2 expression was similar in P6 and adult retina but 22.6 fold lower than in adult pancreas. B–F. P1 rat pups were injected intravitreously with streptozotocin (STZ) or with the citrate buffer vehicle (CTL). B. Retinal flatmounts of P6 CTL or STZ animals were stained using anti-collagen IV antibody. C Representative automated analysis of vascular density using Metamorph software. Branch points are represented by green points and tubes by solid gray lines (lower panel). D–G. Vascular area, mean tube length, branch point density and total tube length density were determined at P6 in hyperglycemic (black bars) or control (white bars) animals. Values in histograms D–G are mean ± SEM of retinas from 8 animals per group from at least 2 different experiments. Data were analyzed by unpaired t-tests. No significant differences were found in these parameters.

From: Kermorvant-Duchemin E, Pinel AC, Lavalette S, Lenne D, Raoul W, Calippe B, et al. (2013) Neonatal Hyperglycemia Inhibits Angiogenesis and Induces Inflammation and Neuronal Degeneration in the Retina.
PLoS ONE 8(11): e79545.

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Published customer image:
Goat anti Human collagen IV antibody used for the demonstration of rat retinal vasculature by immunofluorescence.
Image caption:
Retinal angiogenesis is inhibited in hyperglycemic animals. P1 rat pups were injected with low doses of streptozotocin (STZ) or with the citrate buffer vehicle (CTL). A–B. Retinal flatmounts of P6 CTL (A) or STZ (B) animals were stained using anti-collagen IV antibody. Vascularized area circumferences are highlighted in red. C. Vascular area measurement in hyperglycemic (black bars) or control (white bars) animals. Values in histograms are mean ± SEM of vessel area of retinas from 4–8 animals per group from 3 different experiments. * P<0.05 compared to CTL, two-way ANOVA, Bonferroni post-test. D. Mean tube length, branch point density and total tube length density were determined at P6 in hyperglycemic (black bars) or control (white bars) animals. Values in histograms are mean ± SEM of retinas from 4–8 animals per group from 3 different experiments. E. Higher magnification of P21 retinal flatmounts of CTL and STZ animals stained with collagen IV antibody. F. Mean tube length, branch point density and total tube length density were determined at P21 in hyperglycemic (black bars) or control (white bars) animals. Values in histograms are mean ± SEM of retinas from 4 animals per group from 2 different experiments. Scale bar 1 mm in A–B; 200 μm in E–F. No statistical differences in vessel parameters were found in D and F between STZ and control groups using unpaired t-tests.

From: Kermorvant-Duchemin E, Pinel AC, Lavalette S, Lenne D, Raoul W, Calippe B, et al. (2013) Neonatal Hyperglycemia Inhibits Angiogenesis and Induces Inflammation and Neuronal Degeneration in the Retina.
PLoS ONE 8(11): e79545.

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Goat anti Human collagen IV antibody used for the demonstration of rat retinal vasculature by immunofluorescence.
Image caption:
Figure 4. Astrocytes and pericytes phenotype in NHIR. P1 rat pups were injected with low doses of streptozotocin (STZ) or with the citrate buffer vehicle (CTL). A–D. Retinal flatmounts and sections of P6 CTL (A–B) and STZ (C–D) were co-stained using anti-collagen IV antibody and anti-GFAP antibody. E–F Retinal flatmounts of P6 CTL (E) and STZ (F) were co-stained using anti-collagen IV antibody and or anti-Ng2 antibody. G–H. STZ animals with hyperglycemia >400 mg/DL were co-stained using anti-collagen IV antibody and anti-Ng2 antibody. Nuclei were counterstained with DAPI in A and C. Scale bar 50 μm in A–G, g and g'; 5 μm in E and F insets and H.

From: Kermorvant-Duchemin E, Pinel AC, Lavalette S, Lenne D, Raoul W, Calippe B, et al. (2013) Neonatal Hyperglycemia Inhibits Angiogenesis and Induces Inflammation and Neuronal Degeneration in the Retina.
PLoS ONE 8(11): e79545.

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Goat anti Human collagen IV antibody used for the demonstration of retinal vasculature in mice by immunofluorescence.
Image caption:
Vascular endothelial cell apoptosis and proliferation after BRVO. (A and B) Representative micrographs of TUNEL (green)—CollIV (red) double labeled retinal flatmounts at 1d after BRVO of the occluded vein (A) and the upstream capillary bed (B). (B- right panels) Detail of a DAPI(blue)-labelled upstream capillary depicting a normal nucleus (arrow) and an apoptotic TUNEL+nucleus (arrowhead). (C) Quantification of TUNEL+ECs of control retinas and in the occluded vein and upstream capillary bed at 1, 3 and 7d after BRVO. Values in histograms are mean ± SEM of number of TUNEL+ECs of retina from 5–10 eyes per group. Mann-Whitney non parametric test, * p<0.05 compared to control (0), intact retina or opposite part of the BRVO retina. (D and E) Representative micrographs of EdU (green)—CollIV (red) double labeled retinal flatmounts at 7d after BRVO of the occluded vein (D) and the upstream capillary bed (E). (F) Quantification of EdU positive ECs of control retinas and in the occluded vein and upstream capillary bed at 1, 3 and 7d after BRVO. Mice were daily injected intraperitoneally with EdU at d0 just after laser photocoagulation until sacrifice.Values in histograms are mean ± SEM of number of EdU+ cells of retina from 5–10 eyes per group. Mann-Whitney non parametric test, * p<0.05 compared to control (0), intact retina. Scale bars A, B, D, and E = 100μm, 20µm for insets, B right panels: 10μm.

From: Dominguez E, Raoul W, Calippe B, Sahel J-A, Guillonneau X, Paques M, et al. (2015) Experimental Branch Retinal Vein Occlusion Induces Upstream Pericyte Loss and Vascular Destabilization.
PLoS ONE 10(7): e0132644.

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Published customer image:
Goat anti Human collagen IV antibody used for the demonstration of retinal vasculature in mice by immunofluorescence.
Image caption:
Morphometric analysis of vascular network remodeling after BRVO. (A and B) Representative micrographs of CollIV-labeled retinal flatmounts of a control retina (A) and of the occluded vein after BRVO at 3d (B). Positions of the measurements at 800, 1200 and 1600μm from optic nerve were indicated by arrowhead. (C) Measurements of the retinal vein diameter of control retinas or of non occluded side and in the occluded vein at 1, 3 and 7d and indicated distance from the optic nerve after BRVO. Values in histograms are mean ± SEM of diameter (μm) of control or occluded vein in the retina from 4–11 eyes per group. Mann-Whitney non parametric test, * p<0.05 compared to control (0), non occluded veins of the same eye and intact eyes. (D and E) Representative micrographs of CollIV-labeled retinal flatmounts of the superficial capillary network of a control retina (D) and upstream of the occluded vein at 3d (E). (F and G) Quantification of the surface covered by CollIV-positive capillaries (F) and length (capillary length per surface) at different time points. Values in histograms are mean ± SEM of surface or length of capillaries in the retina from 4–18 eyes per group. Mann-Whitney non parametric test, * p<0.001 compared to control (0), non occluded veins of the same eye and non-occluded eyes. Scale bar = 100μm.

From: Dominguez E, Raoul W, Calippe B, Sahel J-A, Guillonneau X, Paques M, et al. (2015) Experimental Branch Retinal Vein Occlusion Induces Upstream Pericyte Loss and Vascular Destabilization.
PLoS ONE 10(7): e0132644.

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Collagen IV Antibody gallery image 6

Published customer image:
Goat anti Human collagen IV antibody used for the demonstration of retinal vasculature in mice by immunofluorescence.
Image caption:
NG2-staining and pericyte counts after BRVO. (A-D) Representative micrographs of NG2 (green)-CollIV (red) double labeled retinal flatmounts of control (A and B) and at 7d after BRVO (C and D). DAPI is represented in blue in the insets of panel B and D. (E) Quantification of NG2 positive pericytes of control capillaries and capillaries upstream of the occluded vein at indicated time points. Values in histograms are mean ± SEM of number of NG2+ nuclei/mm2 of vascular area. n = 4–18 per group. Mann-Whitney non parametric test, * p<0.05 compared to control (0), intact retina or opposite part of the BRVO retina. Scale bar = 100μm, 25 μm for insets.

From: Dominguez E, Raoul W, Calippe B, Sahel J-A, Guillonneau X, Paques M, et al. (2015) Experimental Branch Retinal Vein Occlusion Induces Upstream Pericyte Loss and Vascular Destabilization.
PLoS ONE 10(7): e0132644.

Enlarge
Collagen IV Antibody gallery image 7

Published customer image:
Goat anti Human collagen IV antibody used for the demonstration of retinal vasculature in mice by immunofluorescence.
Image caption:
Hypoxia induced endothelial cell proliferation in control and BRVO retinas. (A and B) Representative micrographs of EdU (green)—CollIV (red) double-labeled retinal flatmounts at 7d after BRVO raised in normoxia (A) or hypoxia from d3 to d7 (B). All mice were daily injected with EdU from d3 to d7 before sacrifice (please not that retinas in Fig 2 were injected daily with EdU and are therefor not comparable in terms of numbers of EdU+cells). (C) Quantification of EdU positive ECs of the superior vein and upstream capillary bed at d7 of control retinas (without BRVO) and BRVO retinas, raised in normoxia and hypoxia. All mice were daily injected with EdU from d3 to d7 before sacrifice. Values in histograms are mean ± SEM of mRNA expression of occluded area from 9–10 retinas per group. Mann-Whitney non parametric test, ** p<0.01, normoxic BRVO versus hypoxic BRVO. Scale bars AB = 200μm.

From: Dominguez E, Raoul W, Calippe B, Sahel J-A, Guillonneau X, Paques M, et al. (2015) Experimental Branch Retinal Vein Occlusion Induces Upstream Pericyte Loss and Vascular Destabilization.
PLoS ONE 10(7): e0132644.

Enlarge
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  • Goat anti Human Collagen IV
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    134001C, E, IB, IFdatasheet pdfdatasheet pdf0.2 mg
    134001
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human collagen IV antibody recognizes human collagen type IV. It has been cross adsorbed against collagen types I, II, III, V and VI, resulting in <10% cross-reactivity. It may cross-react with collagen type IV from other species.

      Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Collagen Type IV forms a two-dimensional reticulum and is a major component of the basal lamina.

      Goat anti human collagen IV has been successfully employed for the demonstration of vasculature in developing rat pups using immunofluorescence techniques (Kermorvant-Duchemin et al. 2013).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/4000
      Immunoblotting
      Immunofluorescence
      Immunohistology - Frozen1/101/40

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Collagen IV Antibody Formats

    Formats Applications Sizes available
    Collagen IV Antibody : Purified C, E, IB, IF 0.2 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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