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Collagen III Antibody

Collagen III Antibody gallery image 1

Staining of formalin fixed, paraffin embedded human testis with Rabbit anti Human collagen III (AHP1848)

Collagen III Antibody gallery image 2

Staining of formalin fixed, paraffin embedded human kidney with Rabbit anti Human collagen III (AHP1848)

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  • Rabbit anti Human Collagen III
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1848E, P *, WB*datasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human Collagen III antibody recognises Human Collagen III (COL3A1), a secreted protein of 1466 amino acids belonging to the fibrillar collagen family.

      Collagens are highly conserved across species and are vital in the formation of connective tissues. Collagen III is found in most soft connective tissues along with Collagen I.

      Defects in collagen III are associated with Ehlers-Danlos syndrome type 3 and type 4 and with abdominal aortic aneurysm (Treska et al. 2000).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human Collagen III were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.01% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to Human collagen III.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      pH8.0, 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin(1)2.5ug/ml
      Western Blotting(2)1/50001/10000
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
      This product is suitable for use in Western Blotting under non-reducing conditions.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human kidney
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Collagen III Antibody Formats

    Formats Applications Sizes available
    Collagen III Antibody : Purified E, P *, WB* 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Histar Detection SystemSTAR3000A50 TestsC, P
          Antigen Retrieval Buffer, pH4.8BUF027A500 mlP

          Recommended Positive Controls

            Histology Controls

              Human kidney
                • Product Images

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