Collagen II Antibody | COLL-II

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  • Mouse anti Human Collagen II
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    COLL-II
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    2150-0040E, IF*, WBdatasheet pdfdatasheet pdf0.1 ml
    2150-0040
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Collagen II antibody, clone COLL-II recognizes human collagen type II, the main collagen in cartilaginous tissue. Mouse anti Collagen II antibody, clone COLL-II reacts with both pepsin solubilised and CNBr-cleaved human and bovine collagen type II. No-cross reactivity is seen with types I, III, V or IX.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Bovineyes
      Sheepyes
      Mouseyes
      Dogyes
      Pigyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Ascites - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      None present
    • Immunogen
      Human (cartilage specific) CNBr-cleaved collagen 2.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/500
      Western Blotting1/10001/2000
      Immunofluorescence(1)Neat1/5
      (1)
      Suitable for use on fresh, frozen or acetone fixed material. Mild pepsin digestion is recommended to enhance staining.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Collagen II Antibody Formats

    Formats Clone Applications Sizes available
    Collagen II Antibody : Ascites COLL-II E, IF*, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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