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Collagen I/II/III Antibody | NLI/22

Collagen I/II/III Antibody | NLI/22 gallery image 1

Sandwich ELISA analysis of human ferritin using Mouse anti Human collagen I/II/III antibody, clone NLI/22 as a capture reagent and biotinylated Mouse anti Human collagen I antibody, (131008) as a detection reagent with native human collagen (2150-0030) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included diluted 1:4

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  • Mouse anti Human Collagen I/II/III
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    2150-2203C, Edatasheet pdfdatasheet pdf1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • 2150-2203 reacts with collagens type I, II and III. It does not react with human collagen types IV, V or VI, human plasma proteins or albumin from various species.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgM - lyophilised
    • Reconstitution
      Use 1 ml sterile distilled water to give a concentration of 0.1 mg/ml.
    • Preparation
      Gel Filtration chromatography
    • Preservative Stabilisers
      None present
    • Immunogen
      Human placental collagen type 1
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Contains dextran, mannitol and salts.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/51/20

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Collagen I/II/III Antibody Formats

    Formats Clone Applications Sizes available
    Collagen I/II/III Antibody : Purified NLI/22 C, E 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)STAR138A1 mlC, E, P, WB
      Human anti Mouse IgM:FITCHCA040F0.1 mgF
      Goat anti Mouse IgM:FITCSTAR86F1 mgF
      Human anti Mouse IgM:HRPHCA040P0.1 mgE
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Goat anti Mouse IgM:RPESTAR86PE0.5 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

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