Cofilin 2 Antibody

Cofilin 2 Antibody gallery image 1

Western blotting analysis of human skeletal muscle lysate (35µg protein in RIPA buffer) with Goat anti Human cofilin 2 antibody (AHP1061) at 0.03μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Cofilin 2 Antibody gallery image 2

Western blotting analysis of rat skeletal muscle lysate (35µg protein in RIPA buffer) using Goat anti Human cofilin 2 antibody (AHP1061) at 0.01 μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

  • Cofilin 2 Antibody thumbnail image 1
  • Cofilin 2 Antibody thumbnail image 2
  • Goat anti Human Cofilin 2 (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1061E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human Cofilin 2 antibody detects an epitope within the C-terminal (CT) of cofilin 2, an ~18 kDa protein involved in the regulation of actin assembly in developing myotubes through the reversible control of actin polymerization and depolymerization. Cofilin 2 is the major component of intranuclear and cytoplasmic actin rods.

      Cofilin 2 is expressed in developing skeletal muscle at early foetal stages through to a young postnatal stage. During postnatal development, cofilin 1 expression in muscle is down-regulated and cofilin 2 becomes the only isoform in mature skeletal muscle. The onset of cofilin 2 expression is coupled with terminal differentiation of muscle cells. It is suggested that cofilin 2 could be associated with actin filaments in muscle cells more efficiently than cofilin 1.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      SheepExpected from Sequence
      DogExpected from Sequence
      PigExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to Cofilin 2 were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide (NaN3)
      0.5%Bovine Serum Albumin
    • Immunogen
      Peptide with sequence C-DIKDRSTLGEK from C-terminal of Cofilin 2.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.010.3ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1061 detects a band of approximately 18kDa in human duodenum, heart and muscle lysates.
    • Instructions For Use

    Additional Cofilin 2 Antibody Formats

    Formats Applications Sizes available
    Cofilin 2 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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