CNTF

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  • Recombinant Human CNTF
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP173E, FN, WBdatasheet pdfdatasheet pdf20 µg
    PHP173
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • This product is a 199 amino acid, Escherichia coli-derived recombinant protein corresponding to mature human ciliary neutotrophic factor (CTNF), a survival factor for various neuronal cell types believed to prevent the degeneration of motor axons after axotomy.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.2ml of 5mM sodium phosphate, pH 7.5.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
    • Preparation
      Purified recombinant CTNF expressed in E. Coli
    • Preservative Stabilisers
      None present.
    • Activity
      The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is 50-150ng/ml.
    • Purity
      >98% by SDS PAGE/HPLC analysis
    • Approx. Protein Concentrations
      Total protein concentration 0.1mg/ml after reconstitution
    • Molecular Weight
      22.7kD
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <1EU/ug
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      extracellular space
      positive regulation of tyrosine phosphorylation of Stat3 protein
      growth
      ciliary neurotrophic factor receptor binding
      interleukin-6 receptor binding
      growth factor activity
      ciliary neurotrophic factor-mediated signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays50150ng/ml
      Western Blotting1.53.0ng/lane

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      Recombinant human CNTF may be used as a standard for ELISA applications with either a purified human CNTF antibody (AHP1277) or a biotinylated human CNTF antibody (AHP1277B).
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Recombinant human CNTF may be used as the positive control for Western Blotting applications with either a purified human CNTF antibody (AHP1277) or a biotinylated human CNTF antibody (AHP1277B).
    • Instructions For Use

    Additional CNTF Formats

    Formats Applications Sizes available
    CNTF : Rec. Protein E, FN, WB 20 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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