CLDN1 Antibody | 1C5-D9

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CLDN1 Antibody | 1C5-D9 gallery image 1

Western blot analysis of CLDN1 expression in PRC/PRF/5 using Mouse anti Human CLDN1:Azide Free (MCA3605Z)

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CLDN1 Antibody | 1C5-D9 gallery image 2

Staining of paraffin embedded human colon with Mouse anti Human CLDN1:Azide Free (MCA3605Z)

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CLDN1 Antibody | 1C5-D9 gallery image 3

Staining of paraffin embedded human small intestine with Mouse anti Human CLDN1:Azide Free (MCA3605Z)

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  • Mouse anti Human CLDN1:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1C5-D9
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3605ZE, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA3605Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CLDN1 antibody, clone 1C5-D9 recognizes human claudin-1, also known as CLDN1 or Senescence-associated epithelial membrane protein. CLDN1 is a 211 amino acid multi-pass transmembrane protein with a predicted molecular mass of ~23 kDa. CLDN1 is a major constituent of tight junction complexes involved in regulation of epithelial permeability.

      Mutations of the CLDN1 gene can lead to the development of Ichthyosis-sclerosing cholangitis neonatal syndrome (NISCH), an autosomal recessive ichthyosis characterized by scalp hypotrichosis, alopecia, ichthyosis, cholangitis and leukocyte vacuolization (Feldmeyer et al. 2006).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Full length recombinant human CLDN1 (amino acids 1-212) with a GST tag.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      tight junction
      identical protein binding
      integral to plasma membrane
      structural molecule activity
      calcium-independent cell-cell adhesion
      interspecies interaction between organisms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA15ug/ml
      Western Blotting110 ug/ml
      Immunohistology - Paraffin(1)110 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human small intestine
    • Immunofluorescence
    • Western Blotting
      MCA3605Z detects a band of approximately 23 kDa in A-431 cell lysates.
    • Instructions For Use

    Additional CLDN1 Antibody Formats

    Formats Clone Applications Sizes available
    CLDN1 Antibody : Preservative Free 1C5-D9 E, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
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      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
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      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human small intestine

              References

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