Chromogranin A Antibody | LK2H10

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Chromogranin A Antibody | LK2H10 gallery image 1

Citrate pretreated paraffin embedded human pancreas stained with Mouse anti Human Chromogranin A (MCA5696) followed by Histar Detection (STAR3000A)

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Chromogranin A Antibody | LK2H10 gallery image 2

Immunoperoxidase staining of a human pancreas cryosection with Mouse anti Human chromogranin A antibody, clone LK2H10 (MCA5696) followed by Histar detection systm (STAR3000). Low power

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Chromogranin A Antibody | LK2H10 gallery image 3

Immunoperoxidase staining of a human pancreas cryosection with Mouse anti Human chromogranin A antibody, clone LK2H10 (MCA5696) followed by Histar detection systm (STAR3000). Medium power

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Chromogranin A Antibody | LK2H10 gallery image 4

Immunoperoxidase staining of a human pancreas cryosection with Mouse anti Human chromogranin A antibody, clone LK2H10 (MCA5696) followed by Histar detection systm (STAR3000). High power

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  • Mouse anti Human Chromogranin A
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    LK2H10
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5696C, P *, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA5696
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Chromogranin A antibody, clone LK2H10 specifically recognizes chromogranin A (CgA), a secretory glycoprotein and member of the chromogranin/secretogranin family, widely expressed by cells of the neuroendocrine system and related tumours, which is used in immunohistochemistry as a valuable diagnostic marker for pheochromocytomas, paragangliomas and carcinoid, pancreatic and prostate tumours.

      CgA constitutes one of the most abundant components of secretory granules, and is the precursor to several functional peptides, including vasostatin and pancreastatin.

      Clone LK2H10 has been shown to be suitable for use on cytospins (Jensen et al. 1990).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Pigyes
      Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Human pheochromocytoma tissue.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the NS-1 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      protein binding
      extracellular region
      regulation of blood pressure
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting
      Immunohistology - Paraffin(1)1/1001/500
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Normal Pancreas
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Chromogranin A Antibody Formats

    Formats Clone Applications Sizes available
    Chromogranin A Antibody : Purified LK2H10 C, P *, WB 0.2 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
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      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
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      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Normal Pancreas
              • Application NameReference Images
                Immunohistology - Paraffin

              References

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