Western blotting analysis of human kidney lysate (35μg protein in RIPA buffer) probed using Goat anti Human chromogranin A antibody (AHP1393) at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Goat anti Human chromogranin A antibody recognises human chromogranin A, a ~50 kDa secretory glycoprotein and member of the chromogranin/secretogranin family, expressed by secretory vesicles of endocrine and neuroendocrine cells.
Chromogranin A is a precursor for the functional peptides pancreastatin, vasostatin, parastatin and catestatin, which act as negative modulators of hormone and neurotransmitter release in the neuroendocrine system. The measurement of elevated serum levels of chromogranin A is used diagnostically for detecting and monitoring carcinoid (neuroendocrine) tumours, and correlates with a poor prognosis in patients with prostate cancer.
Purified IgG - liquid
Antiserum to human chromogranin A was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
0.02% Sodium Azide (NaN3) 0.5% Bovine Serum Albumin
Peptide sequence C-QGAKERAHQQKKH from the internal region of chromogranin A (NP_001266.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP1393 detects a band of approximately 50kDa in human kidney cell lysates.
1. Nobels, F.R. et al. (1997) Chromogranin A as serum marker for neuroendocrine neoplasia: comparison with neuron-specific enolase and the alpha-subunit of glycoprotein hormones.J Clin Endocrinol Metab. 82 (8): 2622-8.