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Chromogranin A Antibody

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Staining of paraffin embedded human pancreas with Sheep anti Human Chromogranin A (2095-0220) following pronase antigen retrieval

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  • Sheep anti Human Chromogranin A
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    2095-0220E, P *datasheet pdfdatasheet pdf1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Sheep anti Human Chromogranin antibodyrecognizes human chromogranin A, a secreted peptide that inhibits glucose-induced insulin release. Chromogranin A is an activator of microglia (Taupenaut et al. 1996) and is notably upregulated and prominantly expressed in the senile plaques associated with Alzheimer's disease (Yasuhara et al. 1994).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      BovineExpected from Sequence
      ChimpanzeeExpected from Sequence
      DogExpected from Sequence
      HorseExpected from Sequence
      PigExpected from Sequence
      MouseExpected from Sequence
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to the amino acids 1 - 15 (lpvnspmnkgdtevm) of the native molecule conjugated to Keyhole limpet Haemocyanin.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      regulation of blood pressure
      extracellular region
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin(1)1/251/100
      This product requires protein digestion pre-treatment of paraffin sections. Pronase is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human pancreas
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Chromogranin A Antibody Formats

    Formats Applications Sizes available
    Chromogranin A Antibody : Purified E, P * 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Human pancreas
                • Product Images

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