CHRNA5 Antibody (PrecisionAbTM Antibody)

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CHRNA5 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with CHRNA5 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 73 second exposure. Arrow points to CHRNA5 (molecular weight 53 kDa)

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  • CHRNA5 Antibody with Control Lysate
  • Rabbit anti CHRNA5
  • CHRNA5 Antibody with Control Lysate
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    Rabbit anti-human CHRNA5 antibody recognizes neuronal acetylcholine receptor subunit alpha-5, also known as cholinergic receptor or neuronal nicotinic, alpha polypeptide-5. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00527WBdatasheet pdfdatasheet pdf100 µl
      VPA00527
      VPA00527KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00527K
      VPA00527KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00527KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human CHRNA5 antibody recognizes CHRNA5 also known as neuronal acetylcholine receptor subunit alpha-5, Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5, or neuronal nicotinic acetylcholine receptor, alpha5 subunit.

        The protein encoded by the CHRNA5 gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in CHRNA5 have been linked to susceptibility to lung cancer type 2 (LNCR2) (provided by RefSeq, Jun 2010).

        Rabbit anti Human CHRNA5 antibody detects a band of 53 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti CHRNA5 detects a band of approximately 53 kDa in A431 cell lysates
        Anti CHRNA5 detects a band of approximately 53 kDa in A431 cell lysates
        Anti CHRNA5 detects a band of approximately 53 kDa in A431 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
        Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody prepared by affinity chromatography on Protein A
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        2% Sucrose
        0.09% Sodium Azide (NaN3)
        2% Sucrose
        0.09% Sodium Azide (NaN3)
        2% Sucrose
      • Immunogen
        Synthetic peptide directed towards the middle region of human CHRNA5
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg A431 lysate lyophilized in RIPA buffer.
        400μg A431 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        nicotinic acetylcholine-gated receptor-channel complex
        behavioral response to nicotine
        acetylcholine receptor activity
        postsynaptic membrane
        cell junction
        nicotinic acetylcholine-activated cation-selective channel activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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