Goat anti Cholesterol 24 hydroxylase antibody recognizes cholesterol 24-hydroxylase (CYP46A1), a ~58 kDa member of the Cytochrome P450 family, expressed in the brain. CYP46A1 converts cholesterol into the biologically active oxysterol and 24(S)-hydroxycholesterol, in the first step of the enzymatic degradation of cholesterol in the brain. CYP46A1 is also thought to play a role in the metabolism of neurosteroids and drugs that can cross the blood-brain barrier and are targeted to the central nervous system.
P450 enzymes are divided into two groups: steroidogenic and xenobiotic. The latter group is comprised of three families 1, 2 and 3. The xenobiotic p450's are involved in most oxidative drug metabolism.
Serum - liquid
Antisera to cholesterol 24-hydroxylase (CT) were raised by repeated immunisations of goats with highly purified antigen.
Sodium Azide (NaN3)
Synthetic peptide corresponding to amino acid sequence 254-270 of human cholesterol 24-hydroxylase protein.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP1254 detects a band of approximately 58kDa in human Alzheimer diseased brain lysates.