Chk2 Antibody | 4B7

//www.bio-rad-antibodies.com/human-chk2-antibody-4b7-mca3753z.html?RenderPageSpecific=1
Chk2 Antibody | 4B7 gallery image 1

Immunofluorescence showing Chk2 in HeLa cells using Mouse anti Human Chk2:Azide Free (MCA3753Z)

Enlarge
Chk2 Antibody | 4B7 gallery image 2

Staining of paraffin embedded human colon with Mouse anti Human Chk2:Azide Free (MCA3753Z)

Enlarge
Chk2 Antibody | 4B7 gallery image 3

Western blot analysis of Chk2 expression in HeLa cell lysate using Mouse anti Human Chk2:Azide Free (MCA3753Z)

Enlarge
Chk2 Antibody | 4B7 gallery image 4

Western blot analysis of HepG2 human hepatocellular carcinoma whole cell lysate probed with Mouse anti Human Chk2 antibody (MCA3753Z) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence

Enlarge
  • Chk2 Antibody | 4B7 thumbnail image 1
  • Chk2 Antibody | 4B7 thumbnail image 2
  • Chk2 Antibody | 4B7 thumbnail image 3
  • Chk2 Antibody | 4B7 thumbnail image 4
  • Mouse anti Human Chk2:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    4B7
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3753ZIF, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA3753Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Chk2 antibody, clone 4B7 recognizes human Serine/threonine-protein kinase Chk2, also known as Chk2, CHK2 checkpoint homolog, Cds1 homolog, Hucds1, hCds1 or Checkpoint kinase 2. Chk2 is a 543 amino acid ~65 kDa nuclear and cytoplasmic phosphoprotein involved in cell cycle arrest, activation of DNA repair and apoptosis (Yang et al. 2002).

      Mutations in the Chk2 gene have been implicated in the presentation of Li-Fraumeni syndrome 2 (LFS2), a diverse range of malignancies presenting at unusually early ages (Lee et al. 2001), susceptibility to develop prostate cancer (PC), usually presenting as an adenocarcinoma developing in the acini of the prostatic ducts (Dong et al. 2003), Osteogenic sarcoma (OSRC) a sarcoma developing in bone forming cells affecting long bone ends (Miller et al. 2002) and Breast cancer (BC) susceptibility (Wang et al. 2015).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 1-544 of human CHEK2
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting
      Immunohistology - Paraffin(1)0.110 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Chk2 Antibody Formats

    Formats Clone Applications Sizes available
    Chk2 Antibody : Preservative Free 4B7 IF, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      HCA037F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      HCA037P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Write your review

              You may also be interested in...