Chk1 Antibody | 2G1D5

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Chk1 Antibody | 2G1D5 gallery image 1

Western blot analysis of Chk1 using HeLa (lane 2) and NIH/3T3 (lane 3) cell extracts probed with Mouse anti Chk1 (MCA6041). Molecular weight marker is shown in lane 1

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  • Chk1 Antibody | 2G1D5 thumbnail image 1
  • Mouse anti Chk1
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  • Product Type
    Monoclonal Antibody
  • Clone
    2G1D5
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6041WBdatasheet pdfdatasheet pdf50 µg
    MCA6041
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Chk1 antibody, clone 2G1D5 recognizes Chk1 also known as cell cycle checkpoint kinase homologue 1, CHK1 or checkpoint kinase-1.

      Chk1, like the other checkpoint kinase Chk2, is a serine/threonine protein kinase that plays a crucial role in cell cycle control. Chk1 becomes phosphorylated and consequently activated in response to DNA damage by the ATR kinase. Upon this activation Chk1 phosphorylates a variety of proteins including p53 and Cdc25, which triggers cell cycle arrest, DNA repair and apoptosis (Bartek and Lukas, 2003).

      Chk1 plays a critical role in preventing aberrant cells from progressing through the cell cycle. Based on this, it is not surprising that Chk1 is of interest in the field of cancer research and as a therapeutic target (Thompson and Eastman, 2013).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Mouse ant Chk1 recognizes a band of ~55 kDa by western blot
    • Instructions For Use

    Additional Chk1 Antibody Formats

    Formats Clone Applications Sizes available
    Chk1 Antibody : Purified 2G1D5 WB 50 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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