CES1 Antibody (PrecisionAbTM Antibody)

CES1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with CES1 antibody followed by detection with Donkey anti Sheep/Goat IgG (Fc):HRP (1/10,000, STAR88P) and visualized on the ChemiDoc™ MP with 37 second exposure. Arrow points to CES1 (molecular weight 62 kDa)

  • CES1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Goat anti CES1
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    Goat anti-human CES1 antibody recognizes liver carboxylesterase 1, also known as acyl coenzyme A:cholesterol acyltransferase, brain carboxylesterase hBr1, carboxylesterase 1 (monocyte/macrophage serine esterase 1), cocaine carboxylesterase, egasyn or human monocyte/macrophage serine esterase 1. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00555WBdatasheet pdfdatasheet pdf100 µl
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      • Goat anti Human CES1 antibody recognizes CES1 also known as acyl coenzyme A:cholesterol acyltransferase, brain carboxylesterase hBr1, carboxylesterase 1 (monocyte/macrophage serine esterase 1), carboxylesterase 2 (liver), cholesteryl ester hydrolase, cocaine carboxylesterase, egasyn, human monocyte/macrophage serine esterase 1, methylumbelliferyl-acetate deacetylase 1, retinyl ester hydrolase or triacylglycerol hydrolase.

        The CES1 gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of CES1 cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for CES1 (provided by RefSeq, Jun 2010).

        Goat anti Human CES1 antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
      • Western Blotting
        Anti CES1 detects a band of approximately 62 kDa in human liver tissue
      • Product Form
        Purified IgG - liquid
      • Preparation
        Goat polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        TRIS buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
      • Immunogen
        Peptide sequence C-NTQAAQKLKDKE
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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