Cebpa Antibody (PrecisionAbTM Antibody)

Cebpa Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with CEBPA antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 14 second exposure. Arrow points to CEBPA (molecular weight 42 kDa)

  • Cebpa Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Rabbit anti Cebpa
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  • Product Type
    PrecisionAb™ Polyclonal
  • Isotype
    Polyclonal IgG
  • Format
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VPA00700WBdatasheet pdfdatasheet pdf100 µl
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    • Rabbit anti Human CEBPA antibody recognizes the CCAAT/enhancer-binding protein alpha, also known as CCAAT/enhancer-binding protein alpha, CEBP, or C/EBP-beta.

      The protein encoded by the CEBPA intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells (provided by RefSeq, Jul 2008).

      Rabbit anti Human CEBPA antibody detects a band of 42 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
    • Western Blotting
      Rabbit anti CEBPA detects a band of approximately 42 kDa in THP-1 cell lysates
    • Product Form
      Purified IgG - liquid
    • Preparation
      Rabbit polyclonal antibody purified by affinity chromatography
    • Buffer Solution
      TRIS buffered glycine
    • Preservative Stabilisers
      0.01% Thiomersal
      <50% Glycerol
    • Immunogen
      Carrier-protein conjugated synthetic peptide encompassing part of the N-terminal region of human CEBPA
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
    • Lysate Reconstitution
    • Storage
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      generation of precursor metabolites and energy
      positive regulation of transcription from RNA polymerase III promoter
      cytokine-mediated signaling pathway
      transcription factor binding
      promoter binding
      sequence-specific enhancer binding RNA polymerase II transcription factor activity
      interspecies interaction between organisms
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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