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CDw108 Antibody | MEM-150
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Attempts to block the MTRAP-Semaphorin-7A interaction has no effect on erythrocyte invasion. (A) Recombinant Semaphorin-7A and MTRAP have no inhibitory effect on erythrocyte invasion. Purified pentamerised Semaphorin-7A or MTRAP were added to P. falciparum erythrocyte invasion assays at concentrations that exceeded the monomeric equilibrium affinity constant by a factor of 10. (B) Monoclonal anti-Semaphorin-7A antibody has no effect on erythrocyte invasion. Monoclonal anti-Semaphorin-7A (MEM-150) and an anti-basigin positive control (MEM-M6/6) were added to P. falciparum erythrocyte invasion assays in increasing concentrations. MEM-M6/6 showed clear inhibition of invasion, whereas MEM-150 had no observable effect. (C) Rabbit polyclonal antibodies against purified monomeric MTRAP and Semaphorin-7A bind native MTRAP and Semaphorin-7A. Erythrocyte ghosts (left blot) and parasite supernatants (right blot) were analysed by Western blot under non-reducing conditions, and detected by incubation with purified polyclonal antibodies, followed by an anti-rabbit-IgG-HRP antibody. The predicted monomer molecular weights of native Semaphorin-7A and MTRAP are 79.3 and 55.6 kDa respectively. The top arrow indicates the dimer and the bottom arrow the monomer of Semaphorin-7A. (D) Polyclonal antibodies against MTRAP and Semaphorin-7A do not inhibit erythrocyte invasion. Purified polyclonal antibodies against MTRAP and Semaphorin-7A were added to P. falciparum erythrocyte invasion assays but did not affect the efficiency of invasion relative to a positive control (anti-AMA-1).
From: Bartholdson SJ, Bustamante LY, Crosnier C, Johnson S, Lea S, et al. (2012) Semaphorin-7A Is an Erythrocyte Receptor for P. falciparum Merozoite-Specific TRAP Homolog, MTRAP. PLoS Pathog 8(11): e1003031.
- Mouse anti Human CDw108
- Mouse anti Human CDw108:FITC
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Product TypeMonoclonal Antibody
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CloneMEM-150
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IsotypeIgM
2 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Mouse anti Human CDw108 antibody, clone MEM-150 recognizes the human CDw108, cell surface antigen, also known as Semaphorin-7A, John-Milton-Hargen human blood group Ag, Semaphorin-K1 or Semaphorin-L. CDw108 is a 666 amino acid ~80 kDa GPI linked glycoprotein containing a single Ig-like C2 type domain and a single SEMA domain.
CDw108 is expressed by erythrocytes, some T cell lines and some activated lymphocytes.
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Intended Use
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Target SpeciesHuman
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Product FormPurified IgM - liquidPurified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
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Reconstitution
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PreparationPurified IgM prepared by ion exchange chromatographyPurified IgM prepared by ion exchange chromatography
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Preservative Stabilisers
0.09% Sodium Azide 0.09% Sodium Azide 1% Bovine Serum Albumin -
Purity
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Approx. Protein ConcentrationsIsotype concentration 1.0mg/mlIg concentration 0.1mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered salinePhosphate buffered saline
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StorageStore at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.18 months from date of despatch.
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GO Termsregulation of inflammatory responsepositive regulation of ERK1 and ERK2 cascadepositive regulation of axon extensionintegrin-mediated signaling pathwayanchored to membraneexternal side of plasma membraneaxon guidancereceptor activitypositive regulation of macrophage cytokine productionprotein bindingimmune responseinflammatory response
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UniProt
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Entrez Gene
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution Flow Cytometry 1/10 1/50 Immunoprecipitation Western Blotting(1) (1)MEM-150 recognizes CDw108 under non-reducing conditions.Application Name Yes No Min Dilution Max Dilution Flow Cytometry Neat - Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Technical Advice
- Recommended Protocol
- Recommended Protocol
- Flow CytometryUse 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
- ELISA
- ELISA
- Immunohistology
- Immunohistology
- Histology Positive Control Tissue
- Histology Positive Control Tissue
- Immunofluorescence
- Immunofluorescence
- Western Blotting
- Western Blotting
- Instructions For Use
- Instructions For Use
Additional CDw108 Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| CDw108 Antibody : Purified | MEM-150 | F, IP, WB* | 0.2 mg |
| CDw108 Antibody : FITC | MEM-150 | F | 0.1 mg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | 1 mg | C, E, WB | STAR87A | |
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | 1 ml | C, E, P, WB | STAR138A | |
| Human anti Mouse IgM:FITC | HCA040F | 0.1 mg | F | HCA040F | |
| Human anti Mouse IgM:HRP | HCA040P | 0.1 mg | E | HCA040P | |
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | 1 mg | E | STAR87P | |
| Goat anti Mouse IgM:RPE | STAR86PE | 0.5 ml | F | STAR86PE |
Recommended Negative Isotype Control
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgM Negative Control | MCA692 | 100 Tests | F | MCA692 |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgM Negative Control:FITC | MCA692F | 0.1 mg | F | MCA692F |
Useful Reagents
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070B | 200 Test | F | BUF070B | |
| Human Seroblock | BUF070A | 50 Test | F | BUF070A |
Recommended Positive Controls
Histology Controls
References
1. Mudad, R. et al. (1995) Evidence that CDw108 membrane protein bears the JMH blood group antigen.Transfusion. 35: 566-70.
2. Bartholdson, S.J. et al. (2012) Semaphorin-7A is an erythrocyte receptor for P. falciparum merozoite-specific TRAP homolog, MTRAP.PLoS Pathog. 8: e1003031.
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Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
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