CDw108 Antibody | MEM-150

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Attempts to block the MTRAP-Semaphorin-7A interaction has no effect on erythrocyte invasion. (A) Recombinant Semaphorin-7A and MTRAP have no inhibitory effect on erythrocyte invasion. Purified pentamerised Semaphorin-7A or MTRAP were added to P. falciparum erythrocyte invasion assays at concentrations that exceeded the monomeric equilibrium affinity constant by a factor of 10. (B) Monoclonal anti-Semaphorin-7A antibody has no effect on erythrocyte invasion. Monoclonal anti-Semaphorin-7A (MEM-150) and an anti-basigin positive control (MEM-M6/6) were added to P. falciparum erythrocyte invasion assays in increasing concentrations. MEM-M6/6 showed clear inhibition of invasion, whereas MEM-150 had no observable effect. (C) Rabbit polyclonal antibodies against purified monomeric MTRAP and Semaphorin-7A bind native MTRAP and Semaphorin-7A. Erythrocyte ghosts (left blot) and parasite supernatants (right blot) were analysed by Western blot under non-reducing conditions, and detected by incubation with purified polyclonal antibodies, followed by an anti-rabbit-IgG-HRP antibody. The predicted monomer molecular weights of native Semaphorin-7A and MTRAP are 79.3 and 55.6 kDa respectively. The top arrow indicates the dimer and the bottom arrow the monomer of Semaphorin-7A. (D) Polyclonal antibodies against MTRAP and Semaphorin-7A do not inhibit erythrocyte invasion. Purified polyclonal antibodies against MTRAP and Semaphorin-7A were added to P. falciparum erythrocyte invasion assays but did not affect the efficiency of invasion relative to a positive control (anti-AMA-1).

From: Bartholdson SJ, Bustamante LY, Crosnier C, Johnson S, Lea S, et al. (2012) Semaphorin-7A Is an Erythrocyte Receptor for P. falciparum Merozoite-Specific TRAP Homolog, MTRAP. PLoS Pathog 8(11): e1003031.

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  • Mouse anti Human CDw108
  • Mouse anti Human CDw108:FITC
  • Mouse anti Human CDw108:FITC
  • Mouse anti Human CDw108
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MEM-150
  • Isotype
    IgM
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1877FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1877F
    MCA1877F, IP, WB*datasheet pdfdatasheet pdf0.2 mg
    MCA1877
    MCA1877FTFdatasheet pdfdatasheet pdf25 µg
    MCA1877FT
    MCA1877TF, IPdatasheet pdfdatasheet pdf25 µg
    MCA1877T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CDw108 antibody, clone MEM-150 recognizes the human CDw108, cell surface antigen, also known as Semaphorin-7A, John-Milton-Hargen human blood group Ag, Semaphorin-K1 or Semaphorin-L. CDw108 is a 666 amino acid ~80 kDa GPI linked glycoprotein containing a single Ig-like C2 type domain and a single SEMA domain.

      CDw108 is expressed by erythrocytes, some T cell lines and some activated lymphocytes.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgM - liquid
      Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgM - liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by ion exchange chromatography
      Purified IgM prepared by ion exchange chromatography
      Purified IgM prepared by ion exchange chromatography
      Purified IgM prepared by ion exchange chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
    • Purity
    • Approx. Protein Concentrations
      Isotype concentration 1.0mg/ml
      Ig concentration 0.1mg/ml
      Ig concentration 0.1mg/ml
      Isotype concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      regulation of inflammatory response
      positive regulation of ERK1 and ERK2 cascade
      positive regulation of axon extension
      integrin-mediated signaling pathway
      anchored to membrane
      external side of plasma membrane
      axon guidance
      receptor activity
      positive regulation of macrophage cytokine production
      protein binding
      immune response
      inflammatory response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunoprecipitation
      Western Blotting(1)
      (1)
      MEM-150 recognizes CDw108 under non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunoprecipitation
      Western Blotting(1)
      (1)
      MEM-150 recognizes CDw108 under non-reducing conditions.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CDw108 Antibody Formats

    Formats Clone Applications Sizes available
    CDw108 Antibody : FITC MEM-150 F 25 µg | 0.1 mg
    CDw108 Antibody : Purified MEM-150 F, IP, WB* 0.2 mg | 25 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

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        Useful Reagents

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          Human SeroblockBUF070B200 TestF
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          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          Recommended Positive Controls

            Histology Controls

              References

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