CDT1 Antibody

CDT1 Antibody gallery image 1

Human brain probed with Goat anti human CDT1 C-terminal (AHP2062)

CDT1 Antibody gallery image 2

Western blotting analysis of human ovary lysate (35μg protein in RIPA buffer) probed with Goat anti Human CDT1 antibody (AHP2062) at 0.05μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

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  • Goat anti Human CDT1 (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2062E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human Cdt1 antibody recognises human DNA replication factor Cdt1, also known as douple parked homolog or DUP. Cdt1 is a 546 amino acid ~60 kDa DNA replication factor present in the nucleus during G1 and early S phase of the cell cycle and degraded during late S, G2 and M phases., Cdt1 is involved in the formation of the DNA pre-replication complex, in conjunction with CDC6 (cell division control protein 6 homolog).

      Mutations in the CDT1 gene have been identified as a cause of Meier-Gorlin syndrome 4 (MGORS4), a disease anomolies in cranial development and facial features (Bicknell et al. 2011).

      Goat anti Human Cdt1 antibody recognizes an epitope within the C-terminal (CT) region of human Cdt1
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human Cdt1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence C-ARLAHQTRAEEGL from the C-Terminal region of Cdt1 (NP_112190.2).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      DNA replication
      S phase of mitotic cell cycle
      DNA replication checkpoint
      DNA binding
      M/G1 transition of mitotic cell cycle
      protein binding
      regulation of transcription involved in G1/S phase of mitotic cell cycle
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1.03.0ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP2062 detects a band of approximately 65kDa in human brain cell lysates.
    • Instructions For Use

    Additional CDT1 Antibody Formats

    Formats Applications Sizes available
    CDT1 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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