Cdk7 Antibody (PrecisionAbTM Antibody)

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Cdk7 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with cdk7 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 19 second exposure. Arrow points to cdk7 (molecular weight 39 kDa)

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  • Cdk7 Antibody with Control Lysate
  • Mouse anti Cdk7
  • Cdk7 Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
    Cdk7 antibody recognizes cyclin-dependent kinase 7, also known as 39 kDa protein kinase, p39 Mo15, Cdk-activating kinase 1, cell division protein kinase 7, serine/threonine-protein kinase 1 or TFIIH basal transcription factor complex kinase subunit. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2b
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00023WBdatasheet pdfdatasheet pdf100 µl
      VMA00023
      VMA00023KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00023K
      VMA00023KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00023KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human cdk7 antibody recognizes human cyclin-dependent kinase 7, also known as 39 kDa protein kinase, cell division protein kinase 7, serine/threonine-protein kinase 1 and TFIIH basal transcription factor complex kinase subunit.

        Cdk7 is a 346 amino acid member of the CDC2/CDKX subfamily of serine/threonine family of protein kinases. Cdk7, as part of the CAK complex is involved with the transcription factor TFIIH and is thought to be involved in the control of cell cycle progression, DNA repair and RNA polymerase II (pol II) transcription. Cdk7 demonstrates ubiquitous muclear expression in normal tissues and is expressed in cancer tissues (Bartkova et al. 1996).

        Mouse anti Human cdk7 recognizes human cyclin-dependent kinase 7 as a single band of ~39 kDa in multiple human cell lysates by western blotting under reducing conditions.
      • Target Species
        Human
      • Western Blotting
        Anti cdk7 antibody detects a band of approximately 39 kDa in HeLa cell lysates.
        Anti cdk7 antibody detects a band of approximately 39 kDa in HeLa cell lysates.
        Anti cdk7 antibody detects a band of approximately 39 kDa in HeLa cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant
        Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A/G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Recombinant fragment of cdk7 corresponding to a 221 amino acid sequence from the C-terminus
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HeLa lysate lyophilized in RIPA buffer.
        400μg HeLa lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        transcription initiation from RNA polymerase II promoter
        cell division
        transcription initiation from RNA polymerase I promoter
        transcription-coupled nucleotide-excision repair
        androgen receptor signaling pathway
        mRNA capping
        S phase of mitotic cell cycle
        regulation of cyclin-dependent protein kinase activity
        G1 phase of mitotic cell cycle
        holo TFIIH complex
        positive regulation of viral transcription
        DNA-dependent ATPase activity
        G2/M transition of mitotic cell cycle
        ATP binding
        G1/S transition of mitotic cell cycle
        nucleotide-excision repair, DNA damage removal
        mitochondrion
        viral reproduction
        transcription elongation from RNA polymerase II promoter
        androgen receptor binding
        cyclin-dependent protein kinase activity
        positive regulation of transcription from RNA polymerase II promoter
        transcription elongation from RNA polymerase I promoter
        protein phosphorylation
        termination of RNA polymerase I transcription
        protein C-terminus binding
        RNA polymerase II carboxy-terminal domain kinase activity
        transcription coactivator activity
        cell proliferation
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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