Cdk2 Antibody | 3148

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Human CDK2 detected with Human anti Human CDK2 (HCA063)

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  • Cdk2 Antibody | 3148 thumbnail image 1
  • Human anti Human Cdk2
  • Human anti Human Cdk2
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  • Product Type
    Monoclonal Antibody
  • Clone
    3148
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA063E, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA063
    HCA063AE, WBdatasheet pdfdatasheet pdf50 µg
    HCA063A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Human Cdk2 antibody, clone 3148 recognizes human cyclin-dependent kinase 2 (Cdk2), a member of the serine/threonine kinase family of cyclin-dependent kinases. Cyclin-dependant kinases (CDKs) are a class of enzymes that control cell cycle progression in a variety of organisms. Cdk2 forms active heterodimeric complexes with regulatory subunits, including cyclin A or cyclin E, resulting in the phosphorylation of the retinoblastoma tumor suppressor protein. Its activity is regulated by phosphorylation and CDK inhibitors p21/WAF1 (CDKN1A) and p27/kip1 (CDKN1B).

      Cdk2 is involved in the regulation of G1/S transition and S phase progression of the cell cycle but may also play a role in apoptosis and DNA double strand break repair following ionising radiation.

      Cdk2 can be up-regulated in tumors, including adenomas and breast cancers, particularly in conjunction with cyclin over-expression.
    • Intended Use
    • Target Species
      Human
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
    • Reconstitution
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 0.1 mgReconstitute with 1.0ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Recombinant protein corresponding to amino acids 1-124 of human Cdk2.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
      mitosis
      M/G1 transition of mitotic cell cycle
      identical protein binding
      regulation of DNA replication
      cytosol
      G2/M transition of mitotic cell cycle
      blood coagulation
      DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
      S phase of mitotic cell cycle
      cyclin-dependent protein kinase activity
      positive regulation of cell proliferation
      cell division
      G2 phase of mitotic cell cycle
      G1/S transition of mitotic cell cycle
      Ras protein signal transduction
      traversing start control point of mitotic cell cycle
      cyclin-dependent protein kinase holoenzyme complex
      regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
      DNA replication
      ATP binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/500
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/500
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • ELISA
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      HCA063A detects a band of approximately 35kDa in HCT-116 cell lysates.
    • Western Blotting
      HCA063 detects a band of approximately 35kDa in HCT-116 cell lysates.
    • Instructions For Use
    • Instructions For Use

    Additional Cdk2 Antibody Formats

    Formats Clone Applications Sizes available
    Cdk2 Antibody : Purified 3148 E, WB 50 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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