Cdc2 Antibody (PrecisionAbTM Antibody)

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Cdc2 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with Cdc2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 25 second exposure. Arrow points to Cdc2 (molecular weight 34 kDa)

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  • Cdc2 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Cdc2 Antibody with Control Lysate
  • Mouse anti Cdc2
  • Cdc2 Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
    Cdc2 antibody recognizes cyclin-dependent kinase 1, also known as cell cycle controller CDC2, cell division control protein 2 homolog, cell division cycle 2, G1 to S and G2 to M cell division protein kinase 1 or p34 protein kinase. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00098WBdatasheet pdfdatasheet pdf100 µl
      VMA00098
      VMA00098KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00098K
      VMA00098KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00098KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human Cdc2 antibody recognizes Cdc2, also known as cell cycle controller CDC2, cell division control protein 2 homolog, cell division cycle 2, cell division protein kinase 1 and p34 protein kinase.

        Cdc2 is a highly conserved protein with an important role in cell cycle progression and DNA break repair. Cdc2 overexpression in malignant tumor cells may play a role in tumor progression and be indicative of prognostic. Mouse anti Human Cdc2 antibody detects a band of 34 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti Cdc2 detects a band of approximately 34 kDa in Jurkat cell lysates.
        Anti Cdc2 detects a band of approximately 34 kDa in Jurkat cell lysates.
        Anti Cdc2 detects a band of approximately 34 kDa in Jurkat cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Recombinant human p34 cdc2 fusion protein
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer.
        400μg Jurkat lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        anti-apoptosis
        protein localization to kinetochore
        cell division
        Toll signaling pathway
        insulin receptor signaling pathway
        Ras protein signal transduction
        spindle microtubule
        nerve growth factor receptor signaling pathway
        epidermal growth factor receptor signaling pathway
        toll-like receptor 3 signaling pathway
        toll-like receptor 2 signaling pathway
        mitosis
        cytosol
        MyD88-independent toll-like receptor signaling pathway
        midbody
        MyD88-dependent toll-like receptor signaling pathway
        RNA polymerase II carboxy-terminal domain kinase activity
        ATP binding
        activation of MAPKK activity
        innate immune response
        regulation of transcription involved in G1/S phase of mitotic cell cycle
        axon guidance
        toll-like receptor 4 signaling pathway
        positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
        stress-activated MAPK cascade
        activation of MAPK activity
        anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
        nucleoplasm
        cyclin-dependent protein kinase activity
        toll-like receptor 1 signaling pathway
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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