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Cdc2 Antibody

Cdc2 Antibody gallery image 1

Western blot analysis of (A) NIH/3T3 and (B) A431 whole cell lysates probed with Rabbit anti Cdc2

  • Cdc2 Antibody thumbnail image 1
  • Rabbit anti Cdc2
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2407WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Cdc2 antibody recognizes cell division control protein 2 homolog (Cdc2) also known as cyclin-dependent kinase 1 (CDK1) and p34 protein kinase. Cdc2, a serine/threonine kinase, plays a crucial role in regulation of the eukaryotic cell cycle by phosphorylating proteins involved in DNA replication and other important cell cycle progression processes (Enserink and Kolodner 2010). Binding of cyclins to Cdc2 is essential for its regulation, and the kinase only becomes activated once bound to cyclins (Enserink and Kolodner 2010). Cyclin binding has also been reported to play a role in substrate recruitment (Enserink and Kolodner 2010).

      Cdc2 activity is highly regulated through phosphorylation by the serine/threonine protein kinase Wee1 (Harvey et al. 2005). This phosphorylation results in Cdc2 inactivation and consequently, cell cycle arrest at the G2/M transition stage (Harvey et al. 2005). Wee1 phosphorylation is counteracted by the Cdc25 phosphatase, which dephosphorylates Cdc2 thereby enabling entry into mitosis (Harvey et al. 2005).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/400

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti Cdc2 recognizes a band of approximately 34 kDa when run under reducing conditions
    • Instructions For Use

    Additional Cdc2 Antibody Formats

    Formats Applications Sizes available
    Cdc2 Antibody : Purified WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Cdc2 Antibody thumbnail image 1

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