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CD99 antibody | DN16

Product Code Applications Pack Size List Price Your Price Qty
MCA1850
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F WB 0.2 mg loader
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loader

Mouse anti human CD99 antibody, clone DN16 recognizes human CD99, also known as E2 antigen, MIC2 or 12E7. CD99 is a 185 amino acid ~32 kDa single pass type I transmembrane O-glycosylated glycoprotein. Three isoforms can be producted by alternative splicing. Epitope analysis of the DN16 clone suggests the antibody recognizes a minimal peptide sequence "LPDNENKK" located between residues 32 and 39 towards the N-terminal region of the molecule. This sequence is present in both isoforms I and II but is largely absent from isoform 3 suggesting that the antibody will only recognize isoforms I and II (Gil et al. 2002).

CD99 expression is notable in the testis, pancreas, bone marrow, lymph nodes and spleen. CD99 is expressed on all classes of leukocytes and tends to be highest on immature cells.

Functionally CD99 has been found to be involved in cellular adhesion/aggregation (Krisanaprakornkit et al. 2013) and apoptosis (Sciandra et al. 2014).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

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Description Mouse IgG1 Negative Control

References for CD99 antibody

  1. Hahn, J.H. et al. (1997) CD99 (MIC2) regulates the LFA-1/ICAM-1-mediated adhesion of lymphocytes, and its gene encodes both positive and negative regulators of cellular adhesion.
    J Immunol. 159 (5): 2250-8.
  2. Choi, E.Y. et al. (1998) Engagement of CD99 induces up-regulation of TCR and MHC class I and II molecules on the surface of human thymocytes.
    J Immunol. 161 (2): 749-54.
  3. Kim, S.H. et al. (1998) Generation of cells with Hodgkin's and Reed-Sternberg phenotype through downregulation of CD99 (Mic2).
    Blood. 92 (11): 4287-95.
  4. Kim, S.H. et al. (2008) Viral latent membrane protein 1 (LMP-1)-induced CD99 down-regulation in B cells leads to the generation of cells with Hodgkin's and Reed-Sternberg phenotype.
    Blood. 95: 294-300.
  5. Husak, Z. et al. (2010) Death induction by CD99 ligation in TEL/AML1-positive acute lymphoblastic leukemia and normal B cell precursors.
    J Leukoc Biol. 88: 405-12.
  6. Husak, Z. and Dworzak, M.N. (2012) CD99 ligation upregulates HSP70 on acute lymphoblastic leukemia cells and concomitantly increases NK cytotoxicity.
    Cell Death Dis. 3: e425.
  7. Hughes, S.F. et al. (2020) The role of phagocytic leukocytes following flexible ureterenoscopy, for the treatment of kidney stones: an observational, clinical pilots-study.
    Eur J Med Res. 25 (1): 68.

Functional Assays

Synonyms
MIC2
RRID
AB_323201
UniProt
P14209
Entrez Gene
CD99
GO Terms
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0005737 cytoplasm

MCA1850

149255 156149 180605

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