CD95

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  • Recombinant Human CD95 (soluble)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP192FNdatasheet pdfdatasheet pdf25 µg
    PHP192
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • CD95, also known as Fas/APO-1, is a death-domain (DD) containing type I membrane glycoprotein and member of the TNF receptor superfamily, expressed by activated T and B cells, neutrophils, monocytes and thymocytes, which binds to its ligand CD95L (FasL/CD178) to mediate apoptosis.

      The soluble form of CD95 (sCD95), generated by alternative splicing of CD95 mRNA, has the ability to inhibit target cell apoptosis by neutralizing CD95L. Elevated levels of sCD95 have been detected in patients with systemic lupus erythematosus (SLE), and have been associated with a poor prognosis in renal cell cancer and melanoma patients.

      PHP192 can be used in functional studies to inhibit sCD95/CD95L mediated lysis of CD95 sensitive cells.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified recombinant protein - liquid
    • Reconstitution
    • Preparation
      Human CD95 extracellular domain (aa1-154) fused to human Fc-IgG1 produced in human embryo kidney cells.
    • Preservative Stabilisers
      None present
    • Purity
      >95% by SDS PAGE
    • Approx. Protein Concentrations
      1.0mg/ml
    • Molecular Weight
      60kDa under reducing conditions
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays20100ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD95 Formats

    Formats Applications Sizes available
    CD95 : Rec. Protein FN 25 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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