Recombinant soluble Human CD95 corresponds to the spliced soluble form of human CD95. CD95, also known as Fas/APO-1, is a death-domain (DD) containing type I membrane glycoprotein and member of the TNF receptor superfamily, expressed by activated T and B cells, neutrophils, monocytes and thymocytes, which binds to its ligand CD95L (FasL/CD178) to mediate apoptosis.
The soluble form of CD95 (sCD95), generated by alternative splicing of CD95 mRNA, has the ability to inhibit target cell apoptosis by neutralizing CD95L. Elevated levels of sCD95 have been detected in patients with systemic lupus erythematosus (SLE), and have been associated with a poor prognosis in renal cell cancer and melanoma patients.
Recombinant soluble Human CD95 (PHP192) can be used in functional studies to inhibit sCD95/CD95L mediated lysis of CD95 sensitive cells.
N.B. Antibody reactivity and working conditions may vary between species.
Purified recombinant protein - liquid
Human CD95 extracellular domain (aa1-154) fused to human Fc-IgG1 produced in human embryo kidney cells.
>95% by SDS PAGE
Approx. Protein Concentrations
60kDa under reducing conditions
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.