CD92 Antibody | VIM-15b

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  • Mouse anti Human CD92:Alexa Fluor® 647
  • Mouse anti Human CD92:Alexa Fluor® 488
  • Mouse anti Human CD92
  • Mouse anti Human CD92
  • Mouse anti Human CD92:Alexa Fluor® 488
  • Mouse anti Human CD92:FITC
  • Mouse anti Human CD92:FITC
  • Mouse anti Human CD92
  • Mouse anti Human CD92:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    VIM-15b
  • Isotype
    IgG2b
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2521GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA2521GA
    MCA2521FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2521F
    MCA2521C, F, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA2521
    MCA2521PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2521PE
    MCA2521A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2521A647
    MCA2521A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2521A488
    MCA2521FTFdatasheet pdfdatasheet pdf25 µg
    MCA2521FT
    MCA2521TC, F, IPdatasheet pdfdatasheet pdf25 µg
    MCA2521T
    MCA2521A488TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2521A488T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti human CD92 antibody, clone VIM-15b recognizes the C-terminal variant of human CDw92 (isoform 3), also known as CTL1, a 70kDa multi-pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells.

      CDw92 is a member of the choline transporter-like protein family, so called due to their involvement in the efficient supply/transport of the natural amine choline, a vital cell nutrient required for the synthesis of cell membrane phospholipid components and the neurotransmitter acetylcholine.

      Clone VIM-15b is reported to augment the LPS-induced production of IL-10 by monocyte-derived dendritic cells (Mo-DCs), and the reduced expression of CDw92 by Mo-DCs treated with ionomycin or calcium ionophore, can be reinduced in the presence of IL-10 (Wille, S. et al.). Removal of Sodium Azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Alexa Fluor®647 - liquid
      Purified IgG conjugated to Alexa Fluor®488 - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor®488 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 1.0ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
    • Immunogen
      MV4-11 acute monocyte leukaemia cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05mg/ml
      IgG concentration 0.05mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.05mg/ml
      IgG concentration 0.1mg/ml
      IgG concentration 0.1mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
      plasma membrane
      choline transmembrane transporter activity
      mitochondrial outer membrane
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50Pack Size: 0.2 mg, 25 µg
      1/100Pack Size: 0.1 mg
      1/200
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50Pack Size: 0.2 mg, 25 µg
      1/100Pack Size: 0.1 mg
      1/200
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50Pack Size: 0.2 mg, 25 µg
      1/100Pack Size: 0.1 mg
      1/200
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human tonsil.
    • Histology Positive Control Tissue
      Human tonsil.
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human tonsil.
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD92 Antibody Formats

    Formats Clone Applications Sizes available
    CD92 Antibody : Alexa Fluor® 647 VIM-15b F 100 Tests/1ml
    CD92 Antibody : Alexa Fluor® 488 VIM-15b F 100 Tests/1ml | 25 Tests/0.25ml
    CD92 Antibody : Purified VIM-15b C, F, IP 0.2 mg | 0.1 mg | 25 µg
    CD92 Antibody : FITC VIM-15b F 0.1 mg | 25 µg
    CD92 Antibody : RPE VIM-15b F 100 Tests
    • Copyright © 2016 Bio-Rad

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          Recommended Positive Controls

            Histology Controls

              Human tonsil.
              Human tonsil.
              Human tonsil.

              References

              Further Reading

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