CD82 Antibody | B-L2

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CD82 Antibody | B-L2 gallery image 1

Published customer image:
Mouse anti Human CD82 antibody, clone B-L2 used for the identification of CDclusters in cell membrane sheets by immunofluorescence.
Image caption:
Physical characteristics of tetraspanin clusters. A: JY B cell membrane sheets were stained for CD37, CD81 or CD82 and imaged by STED microscopy. Right images: magnification of the area indicated in left images. Scale bars represent 5?μm in whole sheet images and 0.5?μm in zoomed images. B: The diameter of tetraspanin clusters measured from intensity profiles by the full-width at half-maximal intensity (FWHM). At least 120 random clusters were measured derived from at least 3 sheets per condition. Kruskal-Wallis ***. Significance by post hoc analysis is shown (Dunn’s multiple comparison tests between CD53 and other tetraspanins). C: Masks were made for individual CD37 clusters by applying a local threshold on STED images, and of each cluster the circularity was plotted against the area. 405 clusters were analyzed from 3 sheets. Left: dot plot of individual clusters, right: boxplot, Kruskal-Wallis ***, significance by post hoc analysis (Dunn) is shown. D: Density of tetraspanin clusters. For every sheet the analyzed surface area and the number of annotated clusters were determined and the number of clusters per µm2 calculated. At least 7 sheets were analyzed per condition. Kruskal-Wallis ***, significance by post hoc analysis (Dunn) is shown. E: Distances from the center of annotated clusters to the center of their nearest neighbor were determined. The red dashed curves show fits of the nearest neighbor curves with random cluster distributions and the average densities from panel D. Clusters of at least 7 sheets were analyzed per condition.

From: Zuidscherwoude M, Göttfert F, Dunlock VM, Figdor CG, van den Bogaart G, van Spriel AB. The tetraspanin web revisited by super-resolution microscopy.
Sci Rep. 2015 Jul 17;5:12201.

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CD82 Antibody | B-L2 gallery image 2

Published customer image:
Mouse anti Human CD82 antibody, clone B-L2 used for the identification of CDclusters in cell membrane sheets by immunofluorescence.
Image caption:
The tetraspanin web revealed by dual color STED microscopy. A and B: JY B cell membrane sheets double stained for CD53 (red) and CD37, CD81 or CD82 (green) (A), or double stained for CD37 (red) and CD53, CD81 or CD82 (green) (B) were imaged by STED microscopy. Middle images: magnification of the area indicated in whole sheets images (most left). Blob detection images (most right) show annotated clusters used for further analysis. Scale bars represent 5?µm in whole sheet images and 0.5?µm in zoomed and blob detection images. C and D: Percentage of clusters of which the distance from their center to the center of the nearest CD53 (C) or CD37 (D) cluster was within 100?nm. Clusters of at least 7 sheets were analyzed per condition. ANOVA ***, significance by post hoc analysis (Bonferroni) is shown.

From: Zuidscherwoude M, Göttfert F, Dunlock VM, Figdor CG, van den Bogaart G, van Spriel AB. The tetraspanin web revisited by super-resolution microscopy.
Sci Rep. 2015 Jul 17;5:12201.

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CD82 Antibody | B-L2 gallery image 3

Published customer image:
Mouse anti Human CD82 antibody, clone B-L2 used for the identification of CDclusters in cell membrane sheets by immunofluorescence.
Image caption:
Tetraspanin clusters are organized at a higher spatial scale. A and B: Images: zoom of JY B cell membrane sheets double stained for CD53(Rab) (red) and CD53(Mo), CD37, CD81 or CD82 (green) (A) or CD37 (red) and CD53, CD81 or CD82 (green) (B) imaged by STED microscopy. Scale bars represent 0.5?µm. Graphs: intensity profiles of CD53(Rab) or CD37 (red curves) and CD53(Mo), CD37, CD81 or CD82 (filled green) as depicted in the corresponding images. C: Distances from the center of annotated clusters to the center of their nearest CD37 clusters were determined. The nearest neighbor curves were compared to distance curves (red dashed curves) determined from mock images generated with the corresponding cluster densities and sizes, but with completely random and uncorrelated cluster distributions. Clusters of at least 10 sheets were analyzed per condition. D: Zoom of a B cell membrane sheet double stained for CD53 and CD37. Yellow ellipses indicate CD53 and CD37 clusters that are in close proximity to each other. Scale bar represents 1?μm.

From: Zuidscherwoude M, Göttfert F, Dunlock VM, Figdor CG, van den Bogaart G, van Spriel AB. The tetraspanin web revisited by super-resolution microscopy.
Sci Rep. 2015 Jul 17;5:12201.

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  • Mouse anti Human CD82
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  • Product Type
    Monoclonal Antibody
  • Clone
    B-L2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1311C, Fdatasheet pdfdatasheet pdf2 ml
    MCA1311
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD82 antibody, clone B-L2 recognizes human CD82, also known as C33 antigen, Metastasis suppressor Kangai-1, Suppressor of tumorigenicity 6 protein or Tetraspanin-27. CD82 is a 267 amino acid ~50-53 kDa multiple pass transmembrane glycoprotein and member of the tetraspanin family expressed by T cells, NK cells, monocytes, granulocytes and platelets.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by ion exchange chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      CD82 transfected murine mastocytoma cell line P815.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized BALB/c (Iffa Credo) mice were fused with cells of the mouse X63/Ag.8653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      integral to plasma membrane
      protein binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD82 Antibody Formats

    Formats Clone Applications Sizes available
    CD82 Antibody : Purified B-L2 C, F 2 ml
    • Copyright © 2016 Bio-Rad

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              References

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