Published customer image: Rabbit anti Human CD82 antibody used for the detection of CD82 clusters on a B cell membrane by immunofluorescence. Image caption: CD53 does not overlap with CD82. A: B cell membrane sheets double stained for CD82(Rab) (red) and CD82(Mo) (green) were imaged by STED microscopy. Scale bars: 5 μm in whole sheets and 1 μm in zoomed images. Graph: intensity profiles of CD82(Rab) (red curves) and CD82(Mo) (filled green) as depicted in the corresponding images. B: B cells membrane sheets double stained for CD82(Rab) (red) and CD53(Mo) (green) were imaged by STED microscopy. Scale bars: 5 μm in whole sheets and 0.5 μm in zoomed images. Graphs: intensity profiles of CD82 (red line) and CD53 (filled green) as depicted in the corresponding images.
Rabbit anti Human CD82 antibody recognises the carboxy-terminal region of human CD82, also known as KAI1, SAR2 and TSPAN27. CD82 is a ~30 kDa multi-pass transmembrane glycoprotein belonging to the tetraspanin (TM4SF) family.
CD82 acts as a cancer metastasis suppressor protein (Feng et al.2015), while ubiquitously expressed at moderate to high levels in many normal tissues, its expression is down regulated during tumour progression. Loss of CD82 and p53 is correlated with poor prognosis for patients with prostate and other forms of cancer (Liu et al. 2006).
CD82 interacts with CD234 (DARC). Tumour cells dislodged from the primary tumour and expressing CD82 interact with CD234 expressed on vascular cells, transmitting a senescent signal to the tumour cells (Tsai et al 2011). Tumor cells that have lost CD82 expression can proliferate and potentially give rise to metastases (Miranti 2009).
Two isoforms of CD82 may be generated by alternative splicing. The c-terminal region is not affected and thus Rabbit anti Human CD82 antibody is expected to recognize both isoforms of human CD82 (UniProt: P27701).
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Antisera to Human CD82 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
0.02% Sodium Azide (NaN3)
A peptide corresponding to a 15 amino acid sequence close to the carboxy terminus of Human CD82.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Paraffin(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human colon tissue
AHP1709 detects a band of approximately 33kDa in A549 cell lysate
5. Zuidscherwoude, M. et al. (2015) The tetraspanin web revisited by super-resolution microscopy.Sci Rep. 5: 12201.
1. Kauffman, E.C. et al. (2003) Metastasis suppression: the evolving role of metastasis suppressor genes for regulating cancer cell growth at the secondary site.J Urol. 169 (3): 1122-33.
2. Dong, J.T. et al. (1995) KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2.Science. 268 (5212): 884-6.
3. Gil, M.L. et al. (1992) A member of the tetra spans transmembrane protein superfamily is recognized by a monoclonal antibody raised against an HLA class I-deficient, lymphokine-activated killer-susceptible, B lymphocyte line. Cloning and preliminary functional studies.J Immunol. 148 (9): 2826-33.
4. Gaugitsch, H.W. et al. (1991) A new superfamily of lymphoid and melanoma cell proteins with extensive homology to Schistosoma mansoni antigen Sm23.Eur J Immunol. 21 (2): 377-83.