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CD82 Antibody

CD82 Antibody gallery image 1

Western blot analysis of whole cell lysate from A549 human lung adenocarcinoma cells probed with Rabbit anti Human CD82 (AHP1709) at 0.5(A) and 1(B) ug/ml

CD82 Antibody gallery image 2

Immunohistological staining of human colon with Rabbit anti Human CD82 (AHP1709)

CD82 Antibody gallery image 3

Published customer image:
Rabbit anti Human CD82 antibody used for the detection of CD82 clusters on a B cell membrane by immunofluorescence.
Image caption:
CD53 does not overlap with CD82. A: B cell membrane sheets double stained for CD82(Rab) (red) and CD82(Mo) (green) were imaged by STED microscopy. Scale bars: 5 μm in whole sheets and 1 μm in zoomed images. Graph: intensity profiles of CD82(Rab) (red curves) and CD82(Mo) (filled green) as depicted in the corresponding images. B: B cells membrane sheets double stained for CD82(Rab) (red) and CD53(Mo) (green) were imaged by STED microscopy. Scale bars: 5 μm in whole sheets and 0.5 μm in zoomed images. Graphs: intensity profiles of CD82 (red line) and CD53 (filled green) as depicted in the corresponding images.

From: Zuidscherwoude, M. et al. The tetraspanin web revisited by super-resolution microscopy.
Sci. Rep. 5, 12201; doi: 10.1038/srep12201 (2015).

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  • Rabbit anti Human CD82 (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1709P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human CD82 antibody recognises the carboxy-terminal region of human CD82, also known as KAI1, SAR2 and TSPAN27. CD82 is a ~30 kDa multi-pass transmembrane glycoprotein belonging to the tetraspanin (TM4SF) family.

      CD82 acts as a cancer metastasis suppressor protein (Feng et al.2015), while ubiquitously expressed at moderate to high levels in many normal tissues, its expression is down regulated during tumour progression. Loss of CD82 and p53 is correlated with poor prognosis for patients with prostate and other forms of cancer (Liu et al. 2006).

      CD82 interacts with CD234 (DARC). Tumour cells dislodged from the primary tumour and expressing CD82 interact with CD234 expressed on vascular cells, transmitting a senescent signal to the tumour cells (Tsai et al 2011). Tumour cells that have lost CD82 expression can proliferate and potentially give rise to metastases (Miranti 2009).

      Two isoforms of CD82 may be generated by alternative splicing. The c-terminal region is not affected and thus Rabbit anti Human CD82 antibody is expected to recognize both isoforms of human CD82 (UniProt: P27701).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to Human CD82 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
    • Immunogen
      A peptide corresponding to a 15 amino acid sequence close to the carboxy terminus of Human CD82.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      protein binding
      integral to plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.51.0ug/ml
      Immunohistology - Paraffin(1)2.5ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human colon tissue
    • Immunofluorescence
    • Western Blotting
      AHP1709 detects a band of approximately 33kDa in A549 cell lysate
    • Instructions For Use

    Additional CD82 Antibody Formats

    Formats Applications Sizes available
    CD82 Antibody : Purified P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Histar Detection SystemSTAR3000A50 TestsC, P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Human colon tissue
                • Product Images

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