CD81 Antibody | 1D6

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CD81 Antibody | 1D6 gallery image 1

Staining of human peripheral blood lymphocytes with Mouse anti Human CD81 (MCA1847)

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CD81 Antibody | 1D6 gallery image 2

Staining of human peripheral blood lymphocytes with Mouse anti Human CD81: FITC (MCA1847F)

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CD81 Antibody | 1D6 gallery image 3

Staining of human peripheral blood lymphocytes with Mouse anti Human CD81:Low Endotoxin (MCA1847EL)

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  • CD81 Antibody | 1D6 thumbnail image 1
  • CD81 Antibody | 1D6 thumbnail image 2
  • CD81 Antibody | 1D6 thumbnail image 3
  • Mouse anti Human CD81:FITC
  • Mouse anti Human CD81:Low Endotoxin
  • Mouse anti Human CD81
  • Mouse anti Human CD81:FITC
  • Mouse anti Human CD81
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1D6
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1847FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1847F
    MCA1847F, IF, IP, P, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA1847
    MCA1847ELF, FN, IF, IP, P, WBdatasheet pdfdatasheet pdf0.5 mg
    MCA1847EL
    MCA1847FTFdatasheet pdfdatasheet pdf25 µg
    MCA1847FT
    MCA1847TF *, IF, IP, P, WBdatasheet pdfdatasheet pdf25 µg
    MCA1847T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD81 antibody, clone 1D6 recognizes human CD81, a 26kDa cell surface antigen also known as TAPA-1, and a member of the tetraspanin family.

      CD81 is widely expressed on human leucocytes and appears to be involved in a variety of cellular leucocytes including activation, proliferation and differentiation.

      Mouse anti Human CD81 antibody, clone 1D6 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti-proliferative effects.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Chimpanzeeyes
      Sheepyes
      Goatyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 25 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.2 mgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      OCI-LY8 cells aggregated by 5A6 (another CD81 antibody)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      phosphatidylinositol biosynthetic process
      positive regulation of 1-phosphatidylinositol 4-kinase activity
      positive regulation of cell proliferation
      positive regulation of peptidyl-tyrosine phosphorylation
      integral to plasma membrane
      protein binding
      cell proliferation
      protein localization
      regulation of immune response
      activation of MAPK activity
      virion attachment, binding of host cell surface receptor
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      Functional Assays
      Immunofluorescence
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)1/51/10
      Immunofluorescence
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting
      (1)
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)1/51/10
      Immunofluorescence
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting
      (1)
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD81 Antibody Formats

    Formats Clone Applications Sizes available
    CD81 Antibody : Low Endotoxin 1D6 F, FN, IF, IP, P, WB 0.5 mg
    CD81 Antibody : Purified 1D6 F, F *, IF, IP, P, WB 25 µg | 0.2 mg
    CD81 Antibody : FITC 1D6 F 0.1 mg | 25 µg
    • Copyright © 2016 Bio-Rad

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