CD80 Antibody (PrecisionAbTM Antibody) | OTI2B11

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CD80 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI2B11 gallery image 1

Western blot analysis of whole cell lysates probed with CD80 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 60 second exposure. Arrow points to CD80 (molecular weight 60 kDa)

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  • CD80 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI2B11 thumbnail image 1
  • Mouse anti CD80
  • CD80 Antibody with Control Lysate
  • CD80 Antibody with Control Lysate
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    CD80 antibody recognizes the T-lymphocyte activation antigen CD80, also known as B-lymphocyte activation antigen B7, CTLA-4 counter-receptor B7.1, activation B7-1 antigen, or costimulatory molecule variant IgV-CD80. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00138WBdatasheet pdfdatasheet pdf100 µl
      VMA00138
      VMA00138KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00138K
      VMA00138KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00138KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human CD80 antibody recognizes CD80, also known as B-lymphocyte activation antigen B7, CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80, activation B7-1 antigen, costimulatory factor CD80 and costimulatory molecule variant IgV-CD80.

        CD80 is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy (provided by RefSeq, Aug 2011).

        Mouse anti Human CD80 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti CD80 detects a band of approximately 60 kDa in Raji cell lysates.
        Anti CD80 detects a band of approximately 60 kDa in Raji cell lysates.
        Anti CD80 detects a band of approximately 60 kDa in Raji cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography from ascites.
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Full length recombinant human CD80 (NP_005182) produced in HEK293T cells
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Raji lysate lyophilized in RIPA buffer.
        400μg Raji lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        positive regulation of peptidyl-tyrosine phosphorylation
        interspecies interaction between organisms
        transcription activator activity
        cell-cell signaling
        intracellular signal transduction
        protein binding
        positive regulation of interleukin-2 biosynthetic process
        T cell activation
        coreceptor activity
        T cell costimulation
        positive regulation of signal transduction
        positive regulation of T-helper 1 cell differentiation
        positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process
        integral to membrane
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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