CD8/CD38/CD3/HLA DR | LT8 / AT13/5 / UCHT1 / YE2/36-HLK

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CD8/CD38/CD3/HLA DR | LT8 / AT13/5 / UCHT1 / YE2/36-HLK gallery image 1

Human peripheral blood lymphocytes stained with Multi-Colour Human CD8/ CD38/CD3/HLA DR flow kit (4C004)

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  • CD8/CD38/CD3/HLA DR | LT8 / AT13/5 / UCHT1 / YE2/36-HLK thumbnail image 1
  • Four-Color Human CD8/CD38/CD3/HLA DR Flow Kit
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  • Product Type
    Four Color Reagent
  • Clone
    LT8 / AT13/5 / UCHT1 / YE2/36-HLK
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4C004Fdatasheet pdfdatasheet pdf50 Tests/0.5ml
    4C004
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    References
    Reviews
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    • Four-Color Human Flow Kit, CD8/CD38/CD3/HLA DR, clones LT8 / AT13/5 / UCHT1 / YE2/36-HLK can be used for single-step identification of human activated suppressor/cytotoxic (CD3+CD8+CD38+ and CD3+CD8+HLA DR+) T-cell subsets, useful in the study of Acquired Immunodeficiency Syndrome (AIDS) and other viral infection including Epstein Barr Virus (EBV) and Cytomegalovirus (CMV).

      CD3 is a member of the immunoglobulin superfamily, which acts as a mediator of signal transduction, through association with the α/β or γ/δ T-cell receptor (TCR). Mammalian CD3 is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, ζ), consisting of two heterodimers (εγ, εδ) and one homodimer (ζζ). CD3 is expressed by a high-percentage of circulating peripheral T-cells and is considered a pan T-cell marker. Clone UCHT1 specifically recognizes the 20kDa CD3ε chain.

      CD8 is a cell surface glycoprotein which acts as a co-receptor for MHC Class I, in conjunction with the T-cell receptor (TCR). CD8 exists as a dimer, composed of two α chains or more commonly as an αβ heterodimer. The CD8 antigen is expressed on the human suppressor/cytotoxic T-cell subset (CD3+CD8+) and on a subset of NK cells. Binding of CD8 to MHC class I, acts to enhance resting T-cell activation. Clone LT8 is specific for the CD8α chain.

      CD38, otherwise known as cyclic-ADP ribose hydrolase 1, is a type II integral transmembrane glycoprotein and member of the ADP-ribosyl cyclase family, which is widely used to study the processes of B- and T-cell differentiation and activation. An increase in CD8+CD38+ T-cells is a useful indicator of disease progression in HIV infection. This same subset of activated T-cells is also increased in other active viral infections such as EBV and CMV.

      HLA DR is a heterodimeric cell surface glycoprotein and human class II MHC (major histocompatibility complex) cell surface receptor, consisting of a 36kDa alpha and 27kDa beta chain, which is essential for efficient peptide presentation to CD4+ T-cells. HLA DR is expressed primarily by antigen presenting cells and, together with CD38, is a useful marker of T-cell activation following viral infection. Clone YE2/36-HLK recognises a monomorphic determinant of human HLA DR.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD8/CD38/CD3/HLA DR Formats

    Formats Clone Applications Sizes available
    CD8/CD38/CD3/HLA DR : 4 Color LT8 / AT13/5 / UCHT1 / YE2/36-HLK F 50 Tests/0.5ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Flow Cytometry Absolute Count Standard™FCSC58020 TestsF
          FCSC580
          Erythrolyse Red Blood Cell Lysing Buffer (10x)BUF04B100 Test/20mlF
          BUF04B
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          Erythrolyse Red Blood Cell Lysing Buffer (10x)BUF04C500 Tests/100mlF
          BUF04C

          Recommended Positive Controls

            Histology Controls

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