Staining of normal human peripheral blood lymphocytes with DC030. Note differentiation of CD5 positive T cells (strong CD5), CD79a positive B cells and subset of CD5 positive B cells showing expression of CD79a and weak CD5 expression
Mouse anti Human CD79a:FITC/CD5:RPE dual color reagent is intended for the simultaneous evaluation of CD79a and CD5 on human cells.
CD79a is part of the B cell receptor complex, and is expressed by all B lymphocytes.
CD5 is a ~67 kDa glycoprotein expressed by all mature T cells and by a subset of mature B cells. CD5 has been shown to act as a ligand for the B cell marker CD72. CD5 positive B cells have been implicated in the pathogenesis of various autoimmune disorders and are the normal counterpart of the malignant cells in B cell chronic lymphocytic leukaemia.
Mouse anti Human CD79a:FITC/CD5:RPE may find particular application in the study of CD5 positive B lymphocytes and in the study of chronic lymphoproliferative diseases. The CD79a specific antibody in this combination has been reported to be useful in distinguishing B-CLL from mantle cell lymphoma, see (Bell et al. 1999).
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.