Rat anti Human CD77 antibody, clone 38-13 recognizes the Gb3 - specific sugar sequence Gal alpha 1-4 Gal beta- 4G1c, also known as the blood group substance pk (CD77).
CD77 expression amongst leucocytes is restricted to the B cell lineage, and only cells at a certain maturation stage express the antigen.
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgM - liquid
Purified IgM prepared by gel filtration from ascites
Bovine Serum Albumin
Daudi cell line.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Spleen cells from immunised Lewis rats were fused with cells of the SP2/0-Ag14 myeloma cell line.
Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
18 months from date of despatch.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 20ul of the suggested working dilution to label 5 x 105 cells in 100ul