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CD77 Antibody | 38-13

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  • Rat anti Human CD77
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA579Fdatasheet pdfdatasheet pdf100 Tests
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • MCA579 is specific for the Gb3 - specific sugar sequence Gal alpha 1-4 Gal beta- 4G1c, also known as the blood group substance pk (CD77).

      CD77 expression amongst leucocytes is restricted to the B cell lineage, and only cells at a certain maturation stage express the antigen.

      In immunohistology, CD77 stains germinal centre B cells, but not those of the mantle or marginal zone. It is also expressed by follicular dendritic cells, on endothelium and on some epithelial cell types.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgM - liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by gel filtration from ascites
    • Preservative Stabilisers
      0.1%Sodium Azide
      0.2%Bovine Serum Albumin
    • Immunogen
      Daudi cell line
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Lewis rats were fused with cells of the SP2/0-Ag14 myeloma cell line.
    • Storage
      Store at +4oC.


      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 20ul of the suggested working dilution to label 5 x 105 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD77 Antibody Formats

    Formats Clone Applications Sizes available
    CD77 Antibody : Purified 38-13 F 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgM:Alk. Phos.STAR116A0.1 mgE, WB
      Goat anti Rat IgM:Alk. Phos.STAR116AB0.5 mgE, WB
      Goat anti Rat IgM:Biotin3020081 mgC, E, IB
      Goat anti Rat IgM:FITCSTAR116F0.5 mgF
      Goat anti Rat IgM:HRPSTAR116P0.5 mgE, WB
      Goat anti Rat IgM:RPE3020090.5 mgF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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