CD73 Antibody | 2B6

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CD73 Antibody | 2B6 gallery image 1

Western blot analysis of CD73 expression on human mesenchymal stem cell lysates using Mouse anti Human CD73, clone 2B6 (MCA4622GA)

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CD73 Antibody | 2B6 gallery image 2

Published customer image:
Mouse anti Human CD73 antibody, clone 2B6 used for the detection of CD73 fusion proteins in transfected COS-7 cells by imunofluorescence and western blotting.
Image caption:
Characterization of DsRed-NT5E fusion proteins. A. COS-7 cells were transiently transfected with an expression vector for DsRed fused to wild type form of human NT5E (DsRed-hNT5E WT) and fusion proteins were localized by immunofluorescence with antibodies against fluorescent DsRed (green) and native hNT5E (red) proteins, and by internal fluorescence (purple). The merged picture shows obvious overlap of all 3 signals indicating that DsRed-hNT5E fusion protein is intact and contains both antigens. Magnification 400X. B. Immunoblot analysis of total protein extracts from mock-transfected (mock) or transfected COS-7 cells with expression vectors for DsRed monomer and DsRed-hNT5E (DsRed NT5E) fusion protein using DsRed (left) and hNT5E (right) antibodies. Both antibodies detect a protein with a molecular weight of approximately 90 kiloDaltons (black arrowheads, left and right) while DsRed antibody only detects the DsRed monomer (white arrowhead, left) indicating that DsRed-hNT5E fusion is intact and contains both antigens. Total β-actin protein was used as loading control. C. Enzyme histochemistry of mock-transfected or transfected COS-7 cells with expression vectors for DsRed-hNT5E wild type (WT) and mutant (F1–3) proteins using adenosine monophosphate (AMP) as substrate. Only COS–7 cells transfected with the expression vector for DsRed-hNT5E wild type fusion protein displayed AMPase activity, which could be blocked in presence of known NT5E inhibitor α/β-methylene-ADP. Mock-transfected or COS-7 cells transfected with expression vectors for DsRed-hNT5E mutant (F1–3) proteins lack AMPase activity. Magnification 200X.

From: Fausther M, Lavoie EG, Goree JR, Baldini G, Dranoff JA (2014)
NT5E Mutations That Cause Human Disease Are Associated with Intracellular Mistrafficking of NT5E Protein.
PLoS ONE 9(6): e98568.

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  • Mouse anti Human CD73
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  • Product Type
    Monoclonal Antibody
  • Clone
    2B6
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4622GAE, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4622GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD73, clone 2B6 recognises human CD73, otherwise known as ecto-5'-nucleotidase, a GPI-anchored enzyme and member of the 5'-nucleotidase family, which catalyzes the dephosphorylation of 5'-mononucleotides into their corresponding nucleosides, and can also mediate co-stimulatory signals in T cell activation (Dianzani et al. 1993).

      CD73 is expressed by subpopulations of T and B lymphocytes, and increases during lymphocyte development, acting as a lymphocyte maturation marker. A decrease in CD73 activity has been associated with several immunodeficiency disorders including AIDS and Wiskott-Aldrich syndrome, and CD73 is expressed on a variety of lymphomas and leukaemias, including CLL and ALL (Zhao et al. 2011)
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from ascites
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3) 10% Glycerol
    • Immunogen
      Recombinant human CD73 (amino acids 27-252) purified from E. coli.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse FO myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      nucleotide binding
      anchored to membrane
      pyrimidine nucleoside catabolic process
      plasma membrane
      DNA metabolic process
      purine base metabolic process
      cytoplasm
      5'-nucleotidase activity
      pyrimidine base metabolic process
      membrane fraction
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/10001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA4622GA detects a band of approximately 63 kDa in human mesenchymal stem cell lysates.
    • Instructions For Use

    Additional CD73 Antibody Formats

    Formats Clone Applications Sizes available
    CD73 Antibody : Purified 2B6 E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

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          Recommended Positive Controls

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              References

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