CD71 (soluble) Antibody (PrecisionAbTM Antibody)

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CD71 (soluble) Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with CD71 (soluble) antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 132 second exposure. Arrow points to CD71 (molecular weight 90 kDa)

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  • Mouse anti CD71 (soluble)
  • CD71 (soluble) Antibody with Control Lysate
  • CD71 (soluble) Antibody with Control Lysate
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    CD71 antibody recognizes transferrin receptor protein 1, also known as TR, TfR, TfR1, Trfr, T9 p90 or CD71. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00037WBdatasheet pdfdatasheet pdf100 µl
      VMA00037
      VMA00037KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00037K
      VMA00037KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00037KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human CD71 (soluble) antibody recognizes the human soluble transferrin receptor (sTfR) also known as sCD71 or p90. CD71 is a single pass type II membrane glycoprotein of ~90 kDa containing a single protease associated domain. sCD71 forms a homodimeric complex, a truncated form of the membrane bound transferrin receptor lacking the cytoplasmic and transmembrane regions of the molecule (Shih et al. 1990). It is responsible for the uptake of iron into the cell by binding to and internalising iron-bound transferrin (Kogan et al. 2005). After the iron is released into the cell the apotransferrin is relocated to the cell surface, cleaved at Arg-100 and released into the circulation. In healthy adults almost all transferrin receptors exist in the soluble form (Shih et al. 1993). The level of soluble CD71 is used to detect erythropoietin misuse by athletes (Borrione et al. 2007). It is also used to diagnose iron deficiency anemia (Kamer et al. 2012).

        Mouse anti Human soluble CD71 recognizes human both the soluble transferrin receptor and the complex between soluble transferrin receptor and transferrin. The antibody recognizes CD71 as a single band of ~90 kDa in multiple human cell line lysates by western blotting under reducing conditions.
      • Target Species
        Human
      • Western Blotting
        Anti CD71 (soluble) antibody detects a band of approximately 90 kDa in K562 cell lysates.
        Anti CD71 (soluble) antibody detects a band of approximately 90 kDa in K562 cell lysates.
        Anti CD71 (soluble) antibody detects a band of approximately 90 kDa in K562 cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Purified human soluble transferrin receptor
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg K562 lysate lyophilized in RIPA buffer.
        400μg K562 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        endocytosis
        proteolysis
        integral to plasma membrane
        peptidase activity
        transferrin transport
        melanosome
        transmembrane transport
        interspecies interaction between organisms
        endosome
        coated pit
        cellular iron ion homeostasis
        transferrin receptor activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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