CD68 Antibody | Y1/82A

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CD68 Antibody | Y1/82A gallery image 1

Figure A. PE conjugated Mouse anti Human CD14 (MCA1568PE) and Alexa Fluor 647 conjugated Mouse IgG2b isotype control (MCA691A647). Figure B. PE conjugated Mouse anti Human CD14 (MCA1568PE) and Alexa Fluor 647 conjugated Mouse anti Human CD68 (MCA6014A647). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

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CD68 Antibody | Y1/82A gallery image 2

Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and FITC conjugated Mouse IgG2b isotype control (MCA691F). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and FITC conjugated Mouse anti Human CD68 (MCA6014F). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

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CD68 Antibody | Y1/82A gallery image 3

Figure A. Pacific Blue conjugated Mouse anti Human CD14 (MCA1568PB) and RPE conjugated Mouse IgG2b isotype control (MCA691PE). Figure B. Pacific Blue conjugated Mouse anti Human CD14 (MCA1568PB) and RPE conjugated Mouse anti Human CD68 (MCA6014PE). All experiments performed on red cell lysed human blood, fixed and permeabilized with Leucoperm (BUF09) gated on mononuclear cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

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  • Mouse anti Human CD68:Alexa Fluor® 488
  • Mouse anti Human CD68:FITC
  • Mouse anti Human CD68
  • Mouse anti Human CD68:Alexa Fluor® 647
  • Mouse anti Human CD68:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    Y1/82A
  • Isotype
    IgG2b
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6014FF*datasheet pdfdatasheet pdf0.1 mg
    MCA6014F
    MCA6014GAF*datasheet pdfdatasheet pdf0.1 mg
    MCA6014GA
    MCA6014PEF*datasheet pdfdatasheet pdf100 Tests
    MCA6014PE
    MCA6014A488F*datasheet pdfdatasheet pdf100 Tests/1ml
    MCA6014A488
    MCA6014A647F*datasheet pdfdatasheet pdf100 Tests/1ml
    MCA6014A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD68 antibody, clone Y1/82A recognizes the human CD68 cell surface antigen, a 110kDa glycoprotein primarily expressed by macrophages and monocytes.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Alexa Fluor 488 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor 647 - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
    • Immunogen
      Lysosomal contents of lung macrophages
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at +4oC.
      DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
    • Shelf Life
      12 months from date of despatch
      12 months from date of despatch
      12 months from date of despatch
      12 months from date of despatch
      12 months from date of reconstitution
    • GO Terms
      lysosomal membrane
      endosome membrane
      plasma membrane
      membrane fraction
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)1/101/100
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD68 Antibody Formats

    Formats Clone Applications Sizes available
    CD68 Antibody : FITC Y1/82A F* 0.1 mg
    CD68 Antibody : Alexa Fluor® 488 Y1/82A F* 100 Tests/1ml
    CD68 Antibody : Purified Y1/82A F* 0.1 mg
    CD68 Antibody : Alexa Fluor® 647 Y1/82A F* 100 Tests/1ml
    CD68 Antibody : RPE Y1/82A F* 100 Tests
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2b:FITCHCA038F0.1 mgF
      HCA038F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2b:HRPHCA038P0.1 mgE
      HCA038P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:Alexa Fluor® 488MCA691A488100 Tests/1mlF
        MCA691A488
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:FITCMCA691F100 TestsF
        MCA691F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative ControlMCA691100 TestsF
        MCA691
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:Alexa Fluor® 647MCA691A647100 Tests/1mlF
        MCA691A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:RPEMCA691PE100 TestsF
        MCA691PE

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Leucoperm™BUF0950 TestsF
          BUF09

          Recommended Positive Controls

            Histology Controls

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