CD65s Antibody | VIM-2

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CD65s Antibody | VIM-2 gallery image 1

Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:Low Endotoxin (MCA2520EL)

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CD65s Antibody | VIM-2 gallery image 2

Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:Alexa Fluor® 647 (MCA2520A647)

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CD65s Antibody | VIM-2 gallery image 3

Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:FITC (MCA2520F)

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CD65s Antibody | VIM-2 gallery image 4

Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:RPE (MCA2520PE)

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  • Mouse anti Human CD65s:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    VIM-2
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2520FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2520F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD65s antibody, clone VIM-2 selectively recognizes the sialylated form of human CD65, known as CD65s (VIM-2 antigen), a leucocyte carbohydrate antigen expressed by granulocytes, monocytes and leukaemic cells of myelomonocytic lineage.

      CD65s is aberrantly expressed on some acute myeloid leukaemias (AML) and clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34.

      Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation (Lund-Johansen et al. 1992).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
    • Reconstitution
    • Preparation
      Purified IgM prepared by ammonium sulphate precipitation.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
    • Immunogen
      THP1 (human acute monocytic leukaemia cells)
    • Purity
    • Approx. Protein Concentrations
      IgM concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometryneat1/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD65s Antibody Formats

    Formats Clone Applications Sizes available
    CD65s Antibody : FITC VIM-2 F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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