CD61 Antibody | PM6/13

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CD61 Antibody | PM6/13 gallery image 1

Staining of human peripheral blood platelets with Mouse anti Human CD61:Alexa Fluor® 647 (MCA728A647)

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CD61 Antibody | PM6/13 gallery image 2

Staining of human peripheral blood platelets with Mouse anti Human CD61:FITC (MCA728F)

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CD61 Antibody | PM6/13 gallery image 3

Staining of human peripheral blood platelets with Mouse anti Human CD61:RPE (MCA728PE)

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  • CD61 Antibody | PM6/13 thumbnail image 1
  • CD61 Antibody | PM6/13 thumbnail image 2
  • CD61 Antibody | PM6/13 thumbnail image 3
  • Mouse anti Human CD61:Biotin
  • Mouse anti Human CD61
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    PM6/13
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA728BFdatasheet pdfdatasheet pdf0.1 mg
    MCA728B
    MCA728C, F, IP, WB*datasheet pdfdatasheet pdf0.2 mg
    MCA728
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD61 antibody, clone PM6/13 recognizes a 105kD glycoprotein, which is the integrin beta 3 chain. This molecule associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin.

      Mouse anti Human CD61 antibody, clone PM6/13 has been reported to inhibit binding of fibronectin to activated human platelets. In functional studies it can be used to inhibit agonist induced platelet aggregation.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting(1)
      (1)
      Clone PM6/13 recognizes human CD61 under non-reducing conditions.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Bone marrow
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD61 Antibody Formats

    Formats Clone Applications Sizes available
    CD61 Antibody : Purified PM6/13 C, F, IP, WB* 0.2 mg
    CD61 Antibody : Biotin PM6/13 F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
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          Human SeroblockBUF070A50 TestF
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          Recommended Positive Controls

            Histology Controls

              Bone marrow

              References

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