CD60 Antibody | M-T6004

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  • Mouse anti Human CD60
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  • Product Type
    Monoclonal Antibody
  • Clone
    M-T6004
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1314C, F, IPdatasheet pdfdatasheet pdf2 ml
    MCA1314
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human CD60 antibody, clone M-T6004 recognizes the human CD60 antigen, a carbohydrate structure GD3 (Neu5Acα8Neu5Acα3Galβ4GlcCer) modified by a terminal 9-0-acetylated disialosyl group (9-O-acetyl GD3). Mouse anti human CD60 antibody clone M-T6004 does not recognize 7-O-acetyl GD3 or non-acetylated GD3. CD60 has been divided into 3 sub-types depending on the acetylation present. Non acetylated GD3 is termed CD60a and 7-O-acetyl GD3 termed CD60c. 9-O-acetyl GD3 as recognized by clone M-T6004 has been designated CD60b.

      CD60b is present on CD8+ve helper T cells (Rieber and Rank 1994), CD4+ve memory T cells and on activated B cells (Vater et al. 1997). Expression has also been reported on neuroectodermal cells (Carr et al. 1995) and in breast carcinomas (Gocht et al. 1998). CD60b is not expressed by naive T cells (Wada et al. 1998). GD3 has been described as a pro-apoptotic messenger targeting mitochondria in many lymphoid and myeloid cell types (García-Ruiz et al. 2000). Acetylation abrogates the pro-apoptotic activity of GD3 (Malisan et al. 2002) and CD60b protects tumor cells from apoptosis (Kniep et al. 2006).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Rainbow Troutyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgM - liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by gel filtration from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Purified CD60 antigen from PBL; 9-O-acetyl-GD3 from buttermilk
    • Purity
    • Approx. Protein Concentrations
      IgM concentration 100ug/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized BALB/c mice were fused with cells of the P3. X63.Ag.8653 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD60 Antibody Formats

    Formats Clone Applications Sizes available
    CD60 Antibody : Purified M-T6004 C, F, IP 2 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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