CD57 Antibody | TB01

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CD57 Antibody | TB01 gallery image 1

Staining of human peripheral blood lymphocytes with Mouse anti Human CD57 (MCA1305GA)

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CD57 Antibody | TB01 gallery image 2

Staining of human peripheral blood lymphocytes with Mouse anti Human CD57:FITC (MCA1305F)

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CD57 Antibody | TB01 gallery image 3

Staining of paraffin embedded human tonsil with Mouse anti Human CD57 (MCA1305GA)

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  • Product Type
    Monoclonal Antibody
  • Clone
    TB01
  • Isotype
    IgM
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1305GAC, F, Pdatasheet pdfdatasheet pdf0.1 mg
    MCA1305GA
    MCA1305FFdatasheet pdfdatasheet pdf100 Tests
    MCA1305F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD57 antibody, clone TB01 recognizes CD57, also known as HNK-1, an oligosaccharide antigenic determinant present on a variety of polypeptides, lipids and chondroitin sulphate proteoglycans. Its function is poorly understood. CD57 is present on a subset of NK and T cells.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgM - liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by ion exchange chromatography
      Purified IgM prepared by ion exchange chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      Human neuroblastoma cells.
    • Purity
    • Approx. Protein Concentrations
      IgM concentration 0.1 mg/ml
      IgM concentration 1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen1/101/100
      Immunohistology - Paraffin1/101/50
      Immunoprecipitation
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
      *This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but sodium citrate buffer may enhance staining.
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD57 Antibody Formats

    Formats Clone Applications Sizes available
    CD57 Antibody : FITC TB01 F 100 Tests
    CD57 Antibody : Purified TB01 C, F, P 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)STAR138A1 mlC, E, P, WB
      STAR138A
      Human anti Mouse IgM:FITCHCA040F0.1 mgF
      HCA040F
      Human anti Mouse IgM:HRPHCA040P0.1 mgE
      HCA040P
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Goat anti Mouse IgM:RPESTAR86PE0.5 mlF
      STAR86PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgM Negative Control:FITCMCA692F0.1 mgF
        MCA692F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgM Negative ControlMCA692100 TestsF
        MCA692

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              Human tonsil
              • Application NameReference Images
                Flow Cytometry
                Immunofluorescence

              References

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