CD56 Antibody | C5.9

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  • Mouse anti Human CD56:RPE
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  • Product Type
    Monoclonal Antibody
  • Clone
    C5.9
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1437PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1437PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD56 antibody, clone C5.9 recognizes human Neural cell adhesion molecule 1 (NCAM-1), also known as CD56, MSK39 or NKH-1. CD56 isa a 858 amino acid ~120kDa (depending on isoform) single pass type I transmembrane glycoprotein containing 2 fibronectin type-III domains and five Ig-like C2-type domains (UniProt: P13591).

      CD56 is axpressed on all NK-cells and can be used as a pan-NK marker.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.08%Sodium Azide
      0.2%Gelatin
    • Immunogen
      Cell suspension from the acute myelogenous leukemia cell line KG-1a.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c F1 were fused with cells fron the murine myeloma SP2/0 cell line.
    • Storage
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD56 Antibody Formats

    Formats Clone Applications Sizes available
    CD56 Antibody : RPE C5.9 F 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:RPEMCA691PE100 TestsF
        MCA691PE

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

              Further Reading

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