CD56 Antibody | C5.9

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CD56 Antibody | C5.9 gallery image 1

Figure A. FITC conjugated Mouse anti Human CD57 (MCA1305F) and RPE conjugated Mouse IgG2a isotype control (MCA691PE). Figure B. FITC conjugated Mouse anti Human CD57 (MCA1305F) and RPE conjugated Mouse anti Human CD56 (MCA1437PE). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

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  • Mouse anti Human CD56:RPE
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  • Product Type
    Monoclonal Antibody
  • Clone
    C5.9
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1437PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1437PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD56 antibody, clone C5.9 recognizes human Neural cell adhesion molecule 1 (NCAM-1), also known as CD56, MSK39 or NKH-1. CD56 isa a 858 amino acid ~120kDa (depending on isoform) single pass type I transmembrane glycoprotein containing 2 fibronectin type-III domains and five Ig-like C2-type domains (UniProt: P13591).

      CD56 is axpressed on all NK-cells and can be used as a pan-NK marker.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.08%Sodium Azide
      0.2%Gelatin
    • Immunogen
      Cell suspension from the acute myelogenous leukemia cell line KG-1a.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c F1 were fused with cells fron the murine myeloma SP2/0 cell line.
    • Storage
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1:10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD56 Antibody Formats

    Formats Clone Applications Sizes available
    CD56 Antibody : RPE C5.9 F 100 Tests
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:RPEMCA691PE100 TestsF
        MCA691PE

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

              Further Reading

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