Figure A. FITC conjugated Mouse anti Human CD57 (MCA1305F) and RPE conjugated Mouse IgG2a isotype control (MCA691PE). Figure B. FITC conjugated Mouse anti Human CD57 (MCA1305F) and RPE conjugated Mouse anti Human CD56 (MCA1437PE). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.
Mouse anti Human CD56 antibody, clone C5.9 recognizes human Neural cell adhesion molecule 1 (NCAM-1), also known as CD56, MSK39 or NKH-1. CD56 isa a 858 amino acid ~120kDa (depending on isoform) single pass type I transmembrane glycoprotein containing 2 fibronectin type-III domains and five Ig-like C2-type domains (UniProt: P13591).
CD56 is axpressed on all NK-cells and can be used as a pan-NK marker.
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Cell suspension from the acute myelogenous leukemia cell line KG-1a.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Spleen cells from immunized Balb/c F1 were fused with cells fron the murine myeloma SP2/0 cell line.
Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.