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  • Recombinant Human CD54
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP250E, FN, WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • CD54, otherwise known as ICAM-1, is a widely expressed type I integral membrane glycoprotein and member of the immunoglobulin superfamily, which acts as a ligand for the leukocyte integrin LFA-1 (CD11a/CD18) and Mac-1 integrins, and is the major receptor for human rhinovirus. CD54 is highly expressed on activated endothelial cells and plays a role in transendothelial cell migration to sites of acute inflammation, functioning primarily as an adhesion molecule between endothelial cells and leucocytes, following injury or stress. Levels of soluble CD54 in serum, can be potentially used as an indication of infection, inflammation and certain tumours, and as a target for immunosuppression in transplantation.
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.5ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified recombinant CD54 expressed in CHO cells
    • Preservative Stabilisers
      None present
    • Activity
      CD54 supported the adhesion of more than 50% PMA-stimulated HSB2 cells to plates coated at a CD54 concentration of 25ng/ml.
    • Purity
      >98% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      0.1mg/ml after reconstitution
    • Molecular Weight
      49.5kD (453 amino acid residues)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
    • Test Principle
    • Buffer Solution
      10mM Sodium Phosphate
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      extracellular space
      leukocyte migration
      interferon-gamma-mediated signaling pathway
      integral to plasma membrane
      membrane to membrane docking
      T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
      integrin binding
      transmembrane receptor activity
      regulation of immune response
      adhesion to symbiont
      leukocyte cell-cell adhesion
      regulation of leukocyte mediated cytotoxicity
      virion attachment, binding of host cell surface receptor
      positive regulation of cellular extravasation
      heterophilic cell-cell adhesion
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays
      Western Blotting1.53.0ng/lane

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      PHP250 may be used as a standard in ELISA applications involving AHP2183 or AHP2183B.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      PHP250 may be used as a positive control for western blotting applications involving AHP2183 or AHP2183B.
    • Instructions For Use

    Additional CD54 Formats

    Formats Applications Sizes available
    CD54 : Rec. Protein E, FN, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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