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CD54 / ICAM-1 Antibody

CD54 / ICAM-1 Antibody gallery image 1

Western blot analysis of rat kidney tissue lysate (lane 1), 3T3 cell lysate (lane 2) and Jurkat cell lysate (lane 3 & 4) probed with Rabbit anti CD54 / ICAM-1 antibody

  • CD54 / ICAM-1 Antibody thumbnail image 1
  • Rabbit anti CD54 / ICAM-1
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2318WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti CD54 / ICAM-1 antibody recognizes the CD54 antigen also known as intercellular adhesion molecule 1 (ICAM-1). CD54 is a 532 amino acid single pass type I membrane glycoprotein containing five Ig-like C2-type domains. CD54 is widely expressed and acts as a ligand for the leukocyte integrin LFA-1 (CD11a/CD18) and Mac-1 integrins, it is the major receptor for human rhinovirus (Staunton et al. 1989).

      CD54 is highly expressed on activated endothelial cells (Favoloro 1993) and plays a role in transendothelial cell migration to sites of acute inflammation, functioning primarily as an adhesion molecule between endothelial cells and leucocytes, following injury or stress (Manes and Pober 2011). Levels of soluble CD54 in serum (Matalliotakis et al. 2001) or urine (Kim et al. 2006) can be potentially used as an indication of infection, inflammation and certain tumors, and as a target for immunosuppression in transplantation.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.54.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti CD54 / ICAM-1 antibody recognizes a band of approximately 89-92 kDa in rat kidney tissue lysate, 3T3 cell lysate and Jurkat cell lysate
    • Instructions For Use

    Additional CD54 / ICAM-1 Antibody Formats

    Formats Applications Sizes available
    CD54 / ICAM-1 Antibody : Purified WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
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      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • CD54 / ICAM-1 Antibody thumbnail image 1

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