CD54 Antibody

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Paraffin embedded, formalin fixed human breast invasive ductal adenocarcinoma stained with Rabbit anti human CD54 (AHP2183) following enzyme mediated antigen retrieval using proteinase K

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CD54 Antibody gallery image 2

Paraffin embedded, formalin fixed human breast invasive ductal adenocarcinoma stained with Rabbit anti human CD54 (AHP2183) following enzyme mediated antigen retrieval using proteinase K

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  • CD54 Antibody thumbnail image 1
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  • Rabbit anti Human CD54:Biotin
  • Rabbit anti Human CD54
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2183E, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2183
    AHP2183BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP2183B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human CD54 antibody recognizes human CD54, otherwise known as Intercellular adhesion molecule 1 or ICAM-1. CD54 is a 532 amino acid single pass type I membrane glycoprotein containing five Ig-like C2-type domains. CD54 is widely expressed and acts as a ligand for the leukocyte integrin LFA-1 (CD11a/CD18) and Mac-1 integrins, it is the major receptor for human rhinovirus(Staunton et al. 1989).

      CD54 is highly expressed on activated endothelial cells (Favoloro 1993) and plays a role in transendothelial cell migration to sites of acute inflammation, functioning primarily as an adhesion molecule between endothelial cells and leucocytes, following injury or stress (Manes and Pober 2011). Levels of soluble CD54 in serum (Matalliotakis et al. 2001) or urine (Kim et al. 2006), can be potentially used as an indication of infection, inflammation and certain tumour, and as a target for immunosuppression in transplantation.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
    • Antiserum Preparation
      Antiserum to human CD54 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml after reconstitution
      IgG concentration 1.0mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution
      12 months from date of reconstitution
    • GO Terms
      extracellular space
      regulation of immune response
      transmembrane receptor activity
      virion attachment, binding of host cell surface receptor
      integrin binding
      positive regulation of cellular extravasation
      leukocyte cell-cell adhesion
      interferon-gamma-mediated signaling pathway
      integral to plasma membrane
      adhesion to symbiont
      membrane to membrane docking
      heterophilic cell-cell adhesion
      T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
      leukocyte migration
      regulation of leukocyte mediated cytotoxicity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)
      (1)
      This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This biotinylated human CD54 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human CD54 antibody (AHP2183) as the capture reagent and recombinant human CD54 (PHP250) as the standard.
    • ELISA
      This purified human CD54 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human CD54 antibody (AHP2183B) as the detection reagent and recombinant human CD54 (PHP250) as the standard
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD54 Antibody Formats

    Formats Applications Sizes available
    CD54 Antibody : Purified E, P *, WB 0.1 mg
    CD54 Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              References

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