CD54 Antibody | 84H10

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CD54 Antibody | 84H10 gallery image 1

Published customer image:
ICAM-1 activates PKCa but not PKCßII in HMVECLs. A ) At 70% confluence, HMVECLs cells pretreated with 10 ng/ml TNFa to induce ICAM-1 expression. At 24 hrs, the cells were suspended and immunolabeled with anti-ICAM-1 antibodies and examined by flow cytometry for ICAM-1 expression. B–D) HMVECLs were pretreated with TNFa as in panel A. At 24 hrs, the endothelial cells were nonstimulated (NS) or stimulated with a confluent monolayer of anti-ICAM-1-coated beads or the control anti-PECAM-1-coated beads for 20 minutes in B and D or for the times indicated in C. The cells were lysed and the activation of PKCßII and PKCa was examined by western blot with B) anti-phospho-PKCßIIThr641 or anti-PKCßII or C–D) anti-phospho- PKCaThr638 or anti-PKCa. Shown are representative blots. Shown are the mean ± SEM of 3 experiments. NT, nontreated. *, p<0.05 as compared to the nontreated (NT) groups.

From: Abdala-Valencia H, Berdnikovs S, Cook-Mills JM (2012) Vitamin E Isoforms Differentially Regulate Intercellular Adhesion Molecule-1 Activation of PKCa in Human Microvascular Endothelial Cells. PLoS ONE 7(7): e41054.

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  • CD54 Antibody | 84H10 thumbnail image 1
  • Mouse anti Human CD54
  • Mouse anti Human CD54
  • Mouse anti Human CD54:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    84H10
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA532C *, E, F, FN*, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA532
    MCA532FFdatasheet pdfdatasheet pdf100 Tests
    MCA532F
    MCA532TC *, E, F, FN*, IPdatasheet pdfdatasheet pdf25 µg
    MCA532T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human CD54 antibody, clone 84H10 recognizes the D1 domain of ICAM-1.

      It reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1 beta or IFN gamma on other cell types such as fibroblasts and endothelial cells. Detects an antigen of 90kD.

      Mouse anti Human CD54 antibody, clone 84H10 has been reported to block ICAM1 mediated cellular adhesion and block binding of LFA-1 and P. falciparum to ICAM-1.

      Mouse anti Human CD54 antibody, clone 84H10 is routinely tested in flow cytometry on rat splenocytes.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Dogyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from ascites
      Purified IgG prepared by affinity chromatography on Protein A from ascites
      Purified IgG prepared by affinity chromatography from ascites
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.1% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
    • Immunogen
      K562 cell line.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the MOPC 315 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      regulation of leukocyte mediated cytotoxicity
      positive regulation of cellular extravasation
      leukocyte migration
      integrin binding
      extracellular space
      T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
      adhesion to symbiont
      heterophilic cell-cell adhesion
      interferon-gamma-mediated signaling pathway
      leukocyte cell-cell adhesion
      transmembrane receptor activity
      membrane to membrane docking
      integral to plasma membrane
      regulation of immune response
      virion attachment, binding of host cell surface receptor
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow CytometryNeat1/10
      Immunohistology - Paraffin
      Immunoprecipitation5ug / 10e7 cells
      Functional Assays(1)
      Immunohistology - Frozen(2)1/201/50
      (1)
      This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
      (2)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow CytometryNeat1/10
      Immunohistology - Paraffin
      Immunoprecipitation5ug / 10e7 cells
      Functional Assays(1)
      Immunohistology - Frozen(2)1/201/50
      (1)
      This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
      (2)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD54 Antibody Formats

    Formats Clone Applications Sizes available
    CD54 Antibody : Purified 84H10 C *, E, F, FN*, IP 0.2 mg | 25 µg
    CD54 Antibody : FITC 84H10 F 100 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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              References

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